EGF Mouse
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
The EGF is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
EGF was originally isolated from the male mouse submaxillary gland by Cohen in 1962 . The molecule was later sequenced, and the location of three intramolecular disulfide bonds was determined . This discovery marked the beginning of extensive research into the biological functions and applications of EGF.
EGF is a member of a large family of growth factors that share a common structural motif comprising three intramolecular disulfide bonds . It is produced by various cell types, including mammary gland cells, gut epithelial cells, and cells in the nervous system and the kidney . EGF stimulates the proliferation and differentiation of a wide variety of cell types deriving from both ectoderm and mesoderm .
EGF exerts its effects by binding to the epidermal growth factor receptor (EGFR) on the cell surface. This binding triggers a cascade of downstream signaling pathways that ultimately lead to cellular responses such as proliferation, differentiation, and survival . The bioactivity of recombinant mouse EGF (mEGF) has been demonstrated in various cell proliferation assays .
Mouse EGF is widely used in cell culture, differentiation studies, and functional assays . It serves as an essential ingredient in many cell culture media, promoting the growth and maintenance of various cell types . Researchers utilize mEGF to study cellular processes and develop therapeutic strategies for diseases involving abnormal cell growth and differentiation.
Recombinant mouse EGF is produced using bacterial expression systems, such as Escherichia coli, and is optimized for use in research applications . The purity of recombinant mEGF is typically greater than 98%, as determined by SDS-PAGE analysis . Endotoxin levels are kept below 0.01 ng per microgram of mEGF to ensure its suitability for cell culture applications .
The discovery of EGF and its receptor has had a profound impact on the field of cell biology and medicine. Stanley Cohen was awarded the Nobel Prize in Physiology or Medicine in 1986 for his pioneering work on EGF, highlighting the significance of this growth factor in understanding cellular communication and development .
