EFNA5 Human
Description
EFNA5 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 422 amino acids (21-203 a.a.) and having a molecular mass of 48.1kDa. EFNA5 is expressed with a 239 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Ephrin A5, also known as EFNA5, is a member of the ephrin family of proteins. These proteins are crucial for various cellular processes, including cell migration, adhesion, and repulsion. Ephrin A5 is a glycosylphosphatidylinositol (GPI)-anchored ligand that interacts with Eph receptors, a subfamily of receptor tyrosine kinases. This interaction is essential for bidirectional signaling between adjacent cells, which plays a significant role in neuronal, vascular, and epithelial development .
Ephrin A5 is a cell surface protein that contains an ephrin receptor-binding (RBD) domain. It binds promiscuously to Eph receptors on neighboring cells, leading to contact-dependent signaling. This signaling is crucial for various developmental processes, including brain development, where it modulates cell-cell adhesion and repulsion .
The protein is primarily expressed in the human adult brain, heart, spleen, and ovary, as well as in the fetal brain, lung, and kidney . Ephrin A5 is involved in the regulation of glucose-stimulated insulin secretion by mediating communication between pancreatic islet cells .
Recombinant human Ephrin A5 is produced using various expression systems, including HEK293 cells and mouse myeloma cell lines. The recombinant protein is typically purified to high levels of purity, often exceeding 90% as determined by SDS-PAGE under reducing conditions .
The recombinant protein is available in different formulations, including lyophilized powder and solutions in phosphate-buffered saline (PBS). It is essential to store the protein under specific conditions to maintain its stability and activity. For instance, lyophilized proteins are generally stable for up to 12 months when stored at -20 to -80°C, while reconstituted protein solutions can be stored at 4-8°C for 2-7 days .
Recombinant Ephrin A5 is widely used in research to study its role in cellular processes and signaling pathways. It is also used in functional assays to investigate its interaction with Eph receptors and its effects on cell behavior. The protein’s ability to modulate cell-cell communication makes it a valuable tool for studying developmental biology and disease mechanisms .
