EFNA3 Human, Sf9
Sf9, Baculovirus cells.
Ephrin-A3, EFL2, Ehk1-L, EPLG3, LERK3, EPH-related receptor tyrosine kinase ligand 3.
Greater than 90.0% as determined by SDS-PAGE.
Description
EFNA3 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 434 amino acids (23-214aa) and having a molecular mass of 48.7kDa
EFNA3 is fused to a 242 amino acid hIgG-His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
EFNA3 is part of the ephrin (EPH) family. The EPH-related receptors and ephrins constitute the largest subfamily of receptor protein-tyrosine kinases. They are involved in mediating developmental events, particularly in the nervous system and erythropoiesis. Ephrins are classified into two classes, ephrin-A (EFNA) and ephrin-B (EFNB), based on their structural and sequence similarities. The EFNA class ephrins attach to the membrane through a glycosylphosphatidylinositol linkage, while the EFNB class ephrins are transmembrane proteins.
Recombinant human EFNA3, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It consists of 434 amino acids (23-214aa), resulting in a molecular weight of 48.7kDa. The EFNA3 protein is fused to a 242 amino acid hIgG-His-Tag at its C-terminus. Purification is achieved using proprietary chromatographic techniques.
The Fractalkine solution is provided at a concentration of 0.5 mg/ml. It is formulated in a buffer containing 10% Glycerol and Phosphate Buffered Saline (pH 7.4).
The purity of the product is greater than 90%, as determined by SDS-PAGE analysis.
Ephrin-A3, EFL2, Ehk1-L, EPLG3, LERK3, EPH-related receptor tyrosine kinase ligand 3.
Sf9, Baculovirus cells.
ADPQGPGGAL GNRHAVYWNS SNQHLRREGY TVQVNVNDYL DIYCPHYNSS GVGPGAGPGP
GGGAEQYVLY MVSRNGYRTC NASQGFKRWE CNRPHAPHSP IKFSEKFQRY SAFSLGYEFH
AGHEYYYIST PTHNLHWKCL RMKVFVCCAS TSHSGEKPVP TLPQFTMGPN VKINVLEDFE
GENPQVPKLE KSISGLEPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV
TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY
KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV
EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGKHH
HHHH
Product Science Overview
Ephrin A3 is a single, glycosylated polypeptide chain containing 434 amino acids (23-214aa) and has a molecular mass of approximately 48.7 kDa . The recombinant form of Ephrin A3, produced in Sf9 Baculovirus cells, is fused to a 242 amino acid hIgG-His-Tag at the C-terminus and purified using proprietary chromatographic techniques .
Ephrin A3 binds promiscuously to Eph receptors on adjacent cells, leading to contact-dependent bidirectional signaling. This signaling is essential for migration, repulsion, and adhesion during neuronal, vascular, and epithelial development . The signaling pathway downstream of the receptor is referred to as forward signaling, while the pathway downstream of the ephrin ligand is known as reverse signaling .
