EEF2K Human
Eukaryotic Elongation Factor-2 Kinase Human Recombinant
Cat. No.
BT3949
Source
Escherichia Coli.
Synonyms
Eukaryotic Elongation Factor 2 Kinase, Calcium/Calmodulin-Dependent
Eukaryotic Elongation Factor 2 Kinase, EEF-2 Kinase, EC 2.7.11.20, EEF-2K, Calcium/Calmodulin-Dependent
Eukaryotic Elongation Factor-2 Kinase, Calmodulin-Dependent Protein Kinase III,
Eukaroytic Elongation Factor 2 Kinase, Eukaryotic Elongation Factor-2 Kinase, Elongation
Factor-2 Kinase, EC 2.7.11, HSU93850, Eukaryotic elongation factor 2 kinase.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
EEF2K Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 748 amino acids (1-725 a.a) and
having a molecular mass of 84.6kDa.
EEF2K is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Product Specs
Introduction
Eukaryotic elongation factor-2 kinase (EEF2K) is a threonine kinase responsible for regulating protein synthesis. It achieves this by modulating the rate of peptide chain elongation. When activated by upstream kinases such as AMPK or TRPM7, EEF2K phosphorylates a specific site on the elongation factor EEF2. This phosphorylation prevents EEF2 from binding to ribosomes, rendering it inactive and consequently reducing the rate of protein synthesis.
Description
Recombinant Human EEF2K, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 748 amino acids (specifically, amino acids 1 to 725). It possesses a molecular mass of 84.6 kDa. This EEF2K protein is fused to a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques to ensure high purity.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The EEF2K protein solution is provided at a concentration of 0.25 mg/ml. It is formulated in a phosphate-buffered saline solution (pH 7.4) containing 10% glycerol for stability.
Stability
For short-term storage (up to 2-4 weeks), the EEF2K protein solution should be kept at 4°C. For longer storage, it is recommended to freeze the solution at -20°C. To further enhance long-term stability during freezing, consider adding a carrier protein (either 0.1% HSA or BSA). It is important to avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the EEF2K protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Eukaryotic Elongation Factor 2 Kinase, Calcium/Calmodulin-Dependent
Eukaryotic Elongation Factor 2 Kinase, EEF-2 Kinase, EC 2.7.11.20, EEF-2K, Calcium/Calmodulin-Dependent
Eukaryotic Elongation Factor-2 Kinase, Calmodulin-Dependent Protein Kinase III,
Eukaroytic Elongation Factor 2 Kinase, Eukaryotic Elongation Factor-2 Kinase, Elongation
Factor-2 Kinase, EC 2.7.11, HSU93850, Eukaryotic elongation factor 2 kinase.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMADEDLI FRLEGVDGGQ SPRAGHDGDS DGDSDDEEGY FICPITDDPS
SNQNVNSKVN KYYSNLTKSE RYSSSGSPAN SFHFKEAWKH AIQKAKHMPD PWAEFHLEDI ATERATRHRY
NAVTGEWLDD EVLIKMASQP FGRGAMRECF RTKKLSNFLH
AQQWKGASNY VAKRYIEPVD RDVYFEDVRL QMEAKLWGEE YNRHKPPKQV
DIMQMCIIEL KDRPGKPLFH LEHYIEGKYI KYNSNSGFVR DDNIRLTPQA FSHFTFERSG HQLIVVDIQG
VGDLYTDPQI HTETGTDFGD GNLGVRGMAL FFYSHACNRI CESMGLAPFD LSPRERDAVN
QNTKLLQSAK TILRGTEEKC GSPRVRTLSG SRPPLLRPLS ENSGDENMSD
VTFDSLPSSP SSATPHSQKL DHLHWPVFSD LDNMASRDHD HLDNHRESEN SGDSGYPSEK RGELDDPEPR
EHGHSYSNRK YESDEDSLGS SGRVCVEKWN LLNSSRLHLP RASAVALEVQ RLNALDLEKK
IGKSILGKVH LAMVRYHEGG RFCEKGEEWD QESAVFHLEH AANLGELEAI
VGLGLMYSQL PHHILADVSL KETEENKTKG FDYLLKAAEA GDRQSMILVA RAFDSGQNLS PDRCQDWLEA
LHWYNTALEM TDCDEGGEYD GMQDEPRYMM LAREAEMLFT GGYGLEKDPQ RSGDLYTQAA
EAAMEAMKGR LANQYYQKAE EAWAQMEE.
Product Science Overview
Structure and Function
eEF2K is structurally and functionally distinct from other protein kinases. It phosphorylates eukaryotic elongation factor 2 (eEF2) on a conserved threonine residue, which inhibits the function of eEF2. This phosphorylation event is critical because eEF2 is essential for the translocation step during protein synthesis, where it facilitates the movement of the ribosome along the mRNA .
Role in Cellular Processes
eEF2K is involved in various cellular processes, including:
- Regulation of Protein Synthesis: By phosphorylating eEF2, eEF2K inhibits protein synthesis, which is a vital response to cellular stress conditions such as nutrient deprivation and hypoxia .
- Stress Response: eEF2K plays a significant role in the cellular response to stress. For instance, during starvation, eEF2K helps cells survive by shutting down protein synthesis and conserving energy .
- Neurodegenerative Diseases: Increased activity of eEF2K has been observed in the brains of patients with Parkinson’s disease. Inhibition of eEF2K has been shown to reduce alpha-synuclein toxicity, suggesting a potential therapeutic target for neurodegenerative diseases .
Recombinant Human eEF2K
Research and Therapeutic Potential
eEF2K is a target of interest in cancer research due to its role in regulating protein synthesis and cell survival. Inhibitors of eEF2K are being explored as potential therapeutic agents for cancer treatment . Additionally, the role of eEF2K in neurodegenerative diseases highlights its potential as a target for therapeutic intervention in conditions such as Parkinson’s disease .
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