The EEF1A1 gene is located on chromosome 6 in humans and chromosome 9 in mice . This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl-tRNAs to the ribosome during protein synthesis . The protein has several aliases, including EF-Tu, EF1A, and GRAF-1EF .
eEF1A1 is ubiquitously expressed in various tissues, including the brain, placenta, lung, liver, kidney, and pancreas . Its primary function is to catalyze the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis . This process is essential for the accurate and efficient synthesis of proteins.
Beyond its role in translation elongation, eEF1A1 is involved in several other cellular processes, including:
eEF1A1 also interacts with various other proteins and has binding activities, including nucleotide binding, tRNA binding, GTP binding, and protein kinase binding .
Mouse anti-human eEF1A1 antibodies are monoclonal antibodies developed to specifically target the human eEF1A1 protein. These antibodies are used in various research applications, including:
These antibodies are valuable tools for studying the expression, localization, and function of eEF1A1 in different biological contexts.