EEF1A1 Antibody

Eukaryotic Translation Elongation Factor 1 Alpha 1, Mouse Anti Human
Cat. No.
BT16546
Source
Synonyms
Eukaryotic Translation Elongation Factor 1 Alpha 1, EF1A, EEF1A, LENG7, Leukocyte Receptor Cluster (LRC) Member 7, Elongation Factor Tu, Eukaryotic Elongation Factor 1 A-1, Leukocyte Receptor Cluster Member 7, EF-1-alpha-1, EF-Tu, eEF1A-1, CCS-3, CCS3, EE1A1, EEF-1, GRAF-1EF, HNGC:16303, PTI1, Cervical Cancer Suppressor 3, CTCL Tumor Antigen, EF1a-Like Protein, Elongation Factor 1 Alpha Subunit, Elongation Factor 1-Alpha 1, Eukaryotic Translation Elongation Factor 1 Alpha 1-Like 14, Glucocorticoid Receptor AF-1 Specific Elongation Factor, Prostate Tumor-Inducing Protein 1, Translation Elongation Factor 1 Alpha 1-Like 14.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
EEF1A1, an isoform of elongation factor-1 complex's alpha subunit, facilitates the enzymatic delivery of aminoacyl tRNAs to the ribosome. While EEF1A1 is expressed in the brain, placenta, lung, liver, kidney, and pancreas, the alpha 2 isoform is found in the brain, heart, and skeletal muscle.
Physical Appearance
A clear, colorless solution that has undergone sterile filtration.
Formulation
The solution contains 1 mg/ml of EEF1A1 antibody in a buffer of PBS at pH 7.4, supplemented with 10% glycerol and 0.02% sodium azide.
Storage Procedures
For storage up to 1 month, maintain the antibody at 4°C. For extended storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody remains stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
The EEF1A1 antibody has undergone testing using ELISA, Western blot analysis, and flow cytometry to confirm its specificity and reactivity. As applications may vary, it is recommended to optimize the antibody concentration for each specific use to achieve the best results.
Synonyms
Eukaryotic Translation Elongation Factor 1 Alpha 1, EF1A, EEF1A, LENG7, Leukocyte Receptor Cluster (LRC) Member 7, Elongation Factor Tu, Eukaryotic Elongation Factor 1 A-1, Leukocyte Receptor Cluster Member 7, EF-1-alpha-1, EF-Tu, eEF1A-1, CCS-3, CCS3, EE1A1, EEF-1, GRAF-1EF, HNGC:16303, PTI1, Cervical Cancer Suppressor 3, CTCL Tumor Antigen, EF1a-Like Protein, Elongation Factor 1 Alpha Subunit, Elongation Factor 1-Alpha 1, Eukaryotic Translation Elongation Factor 1 Alpha 1-Like 14, Glucocorticoid Receptor AF-1 Specific Elongation Factor, Prostate Tumor-Inducing Protein 1, Translation Elongation Factor 1 Alpha 1-Like 14.
Purification Method
EEF1A1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT23C11AT.
Immunogen
Anti-human EEF1A1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human EEF1A1 protein 1-462 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and k light chain.

Product Science Overview

Introduction

Eukaryotic Translation Elongation Factor 1 Alpha 1 (eEF1A1) is a crucial protein involved in the elongation phase of protein synthesis. It is encoded by the EEF1A1 gene and is expressed across various eukaryotic organisms, including humans and mice .

Gene and Protein Structure

The EEF1A1 gene is located on chromosome 6 in humans and chromosome 9 in mice . This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl-tRNAs to the ribosome during protein synthesis . The protein has several aliases, including EF-Tu, EF1A, and GRAF-1EF .

Expression and Function

eEF1A1 is ubiquitously expressed in various tissues, including the brain, placenta, lung, liver, kidney, and pancreas . Its primary function is to catalyze the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis . This process is essential for the accurate and efficient synthesis of proteins.

Biological Roles and Pathways

Beyond its role in translation elongation, eEF1A1 is involved in several other cellular processes, including:

  • Regulation of transcription (DNA-templated)
  • Cellular response to epidermal growth factor stimulus
  • Regulation of chaperone-mediated autophagy
  • Protein biosynthesis and methylation
  • Neutrophil degranulation

eEF1A1 also interacts with various other proteins and has binding activities, including nucleotide binding, tRNA binding, GTP binding, and protein kinase binding .

Clinical Significance

eEF1A1 has been identified as an autoantigen in 66% of patients with Felty’s syndrome, an autoimmune disorder associated with rheumatoid arthritis . Additionally, it is involved in several pathways related to viral mRNA translation and infectious diseases .

Mouse Anti-Human eEF1A1 Antibody

Mouse anti-human eEF1A1 antibodies are monoclonal antibodies developed to specifically target the human eEF1A1 protein. These antibodies are used in various research applications, including:

  • Western blotting (WB)
  • Immunocytochemistry/Immunofluorescence (ICC/IF)
  • Enzyme-linked immunosorbent assay (ELISA)

These antibodies are valuable tools for studying the expression, localization, and function of eEF1A1 in different biological contexts.

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Eukaryotic Translation Elongation Factor 1 Alpha 1 Human Recombinant
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Source
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