EDF1 Human

Endothelial Differentiation-Related Factor 1 Human Recombinant
Cat. No.
BT6027
Source
Escherichia Coli.
Synonyms
EDF-1, MBF1, Multiprotein-bridging factor 1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

EDF1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 156 amino acids (1-148 a.a.) and having a molecular mass of 17.4 kDa. The EDF1 is fused to 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
EDF1 is a protein that plays a crucial role in the differentiation of endothelial cells, which are the cells that line the inner surface of blood vessels. It acts as a bridge between regulatory proteins and the machinery responsible for gene transcription, thereby influencing the expression of genes involved in endothelial differentiation. EDF1 achieves this by binding to calmodulin, a calcium-binding protein, through its IQ domain. This interaction allows EDF1 to regulate nitric oxide synthase activity by sequestering calmodulin in the cytoplasm. EDF1 is found in various adult tissues, including the liver, heart, adipose tissue, intestine, and pancreas.
Description
This product consists of recombinant human EDF1 protein produced in E. coli bacteria. It is a single, non-glycosylated polypeptide chain comprising 156 amino acids (specifically, amino acids 1 to 148) and has a molecular weight of 17.4 kDa. An 8-amino acid His-Tag is fused to the C-terminus of the protein to facilitate purification using proprietary chromatographic techniques.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation
The product is provided as a solution with a concentration of 0.5 mg/ml. The solution is buffered with 20mM Tris at pH 8 and contains 1mM DTT, 0.1M NaCl, and 20% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept refrigerated at 4°C. For longer storage, it should be frozen at -20°C. To ensure optimal stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is recommended. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the EDF1 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
EDF-1, MBF1, Multiprotein-bridging factor 1.
Source
Escherichia Coli.
Amino Acid Sequence
MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK QHSITKNTAK LDRETEELHH DRVTLEVGKV IQQGRQSKGL TQKDLATKIN EKPQVIADYE SGRAIPNNQV LGKIERAIGL KLRGKDIGKP IEKGPRAKLE HHHHHH.

Product Science Overview

Gene and Protein Structure

The EDF1 gene is located on chromosome 9q34.3 in humans . The gene undergoes alternative splicing, resulting in multiple transcript variants that encode distinct protein isoforms . The EDF1 protein is also known by several aliases, including Multiprotein Bridging Factor-1 (MBF1) and CFAP280 .

Function and Mechanism

EDF1 functions as a transcriptional coactivator, enhancing the DNA-binding activity of various transcription factors such as ATF1, ATF2, CREB1, and NR5A1 . It achieves this by interconnecting the general transcription factor TATA element-binding protein (TBP) with gene-specific activators . This bridging role is essential for modulating the transcription of genes involved in endothelial differentiation .

Additionally, EDF1 regulates nitric oxide synthase activity, likely by sequestering calmodulin in the cytoplasm . This regulation is vital for maintaining endothelial cell function and vascular health.

Biological Significance

EDF1 is implicated in several critical biological processes:

  • Endothelial Cell Differentiation: EDF1 is essential for the differentiation of endothelial cells, which line the interior surface of blood vessels .
  • Lipid Metabolism: The protein plays a role in lipid metabolism, influencing the body’s ability to process and store fats .
  • Cardiomyocyte Hypertrophy: EDF1 is involved in hormone-induced hypertrophy of cardiomyocytes, the muscle cells of the heart .
Clinical Relevance

Mutations or dysregulation of the EDF1 gene can be associated with various diseases. For instance, EDF1 has been linked to Familial Cold Autoinflammatory Syndrome 2 and Retinitis Pigmentosa 41 . Understanding the function and regulation of EDF1 can provide insights into these conditions and potential therapeutic targets.

Research and Applications

Recombinant EDF1 protein is used in research to study its role in cellular processes and disease mechanisms. By using human recombinant EDF1, scientists can investigate its interactions with other proteins and its impact on gene expression and cellular function.

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