ECSIT Human

ECSIT homolog Human Recombinant
Cat. No.
BT5953
Source
E.coli.
Synonyms
ECSIT Homolog (Drosophila), Evolutionarily Conserved Signaling Intermediate In Toll Pathway Mitochondrial, Likely Ortholog Of Mouse Signaling Intermediate In Toll Pathway Evolutionarily Conserved, Protein SITPEC.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ECSIT Human Recombinant produced in E. coli is a single polypeptide chain containing 222 amino acids (19-217) and having a molecular mass of 24.6 kDa.
ECSIT is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
ECSIT homolog (ECSIT) is a ubiquitously expressed protein that plays a crucial role as an adaptor protein in the cytoplasmic signal transduction cascade initiated by Toll receptor activation. ECSIT facilitates the proteolytic activation of MAP3K1. Moreover, ECSIT participates in the BMP signaling pathway. It is essential for normal embryonic development. Initially categorized as a cytoplasmic protein, ECSIT specifically interacts with TNF receptor-associated factor (TRAF)-6 within the TLR pathway. Notably, ECSIT gene knockdown leads to severely compromised complex I assembly and impaired mitochondrial function.
Description
Recombinant human ECSIT, produced in E. coli, is a single polypeptide chain consisting of 222 amino acids (19-217) with a molecular weight of 24.6 kDa. This protein is fused to a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
The ECSIT solution is prepared in a buffer containing 20mM Tris-HCl (pH 8.0), 0.1M NaCl, 1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Exceeds 90% purity as determined by SDS-PAGE analysis.
Synonyms
ECSIT Homolog (Drosophila), Evolutionarily Conserved Signaling Intermediate In Toll Pathway Mitochondrial, Likely Ortholog Of Mouse Signaling Intermediate In Toll Pathway Evolutionarily Conserved, Protein SITPEC.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSGTCGAAL TGTSISQVPL PKDSTGAADP PQPHIVGIQS PDQQAALARH NPARPVFVEG PFSLWLRNKC VYYHILRADL LPPEEREVEE TPEEWNLYYP MQLDLEYVRS GWDNYEFDIN EVEEGPVFAM CMAGAHDQAT MAKWIQGLQE TNPTLAQIPV VFRLAGSTRE LQTSSAGLEE PPLPEDHQEE DDNLQRQQQG QS

Product Science Overview

Structure and Expression

The ECSIT protein is encoded by the ECSIT gene located on chromosome 19 in humans. The recombinant form of ECSIT is typically expressed in Escherichia coli (E. coli) and is often tagged with a polyhistidine (His) tag to facilitate purification. The recombinant human ECSIT protein consists of 201 amino acids and has a predicted molecular mass of approximately 22.9 kDa .

Function and Significance

ECSIT is an adapter protein involved in the Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. These pathways are essential for the activation of NF-kappa-B (NF-κB), a transcription factor that regulates the expression of genes involved in immune and inflammatory responses . ECSIT specifically interacts with TRAF6 (TNF receptor-associated factor 6) and MEKK-1 (MAP3K1), facilitating the activation of NF-κB .

In addition to its role in immune signaling, ECSIT is also involved in mitochondrial function. It is required for the efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase (complex I), which is a critical component of the mitochondrial respiratory chain . Knockdown of ECSIT results in impaired complex I assembly and disturbed mitochondrial function .

Applications and Research

Recombinant human ECSIT protein is widely used in research to study its role in various cellular processes. It is particularly valuable in investigating the mechanisms of immune response and mitochondrial function. The protein is also used in the development of therapeutic strategies targeting immune and inflammatory diseases.

Stability and Storage

Recombinant human ECSIT protein is typically provided as a lyophilized powder and is stable for up to twelve months when stored at -20°C to -80°C under sterile conditions. It is recommended to aliquot the protein to avoid repeated freeze-thaw cycles .

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