EBNA1BP2 Human

EBNA1 Binding Protein 2 Human Recombinant
Cat. No.
BT6061
Source
Escherichia Coli.
Synonyms
Probable rRNA-processing protein EBP2 isoform 2, EBNA1 binding protein 2, EBP2, NOBP, P40, EBNA1BP2, Nucleolar protein p40.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Recombinant Human EBNA1BP2 produced in E. coli is a single polypeptide chain containing 329 amino acids (1-306) and having a molecular mass of 37.2kDa.
EBNA1BP2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
EBNA1BP2, a member of the EBP2 family, plays a crucial role in processing the 27S pre-rRNA. This protein interacts with the Epstein-Barr Virus (EBV) nuclear antigen 1 (EBNA1). The interaction between EBNA1 and EBP2 is vital for the stable segregation of EBV episomes during cell division.
Description
Recombinant Human EBNA1BP2, expressed in E. coli, is a single polypeptide chain comprising 329 amino acids (residues 1-306) with a molecular weight of 37.2kDa. This protein is fused with a 23 amino acid His-tag at its N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution that has been filtered.
Formulation
The EBNA1BP2 solution is provided at a concentration of 0.5mg/ml and is prepared in a buffer containing 20mM Tris-HCl (pH 8.0), 10% glycerol, and 0.4M Urea.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To ensure long-term stability, consider adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
The purity of EBNA1BP2 is greater than 80% as determined by SDS-PAGE analysis.
Synonyms
Probable rRNA-processing protein EBP2 isoform 2, EBNA1 binding protein 2, EBP2, NOBP, P40, EBNA1BP2, Nucleolar protein p40.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDTPPLS DSESESDESL VTDRELQDAF SRGLLKPGLN VVLEGPKKAV NDVNGLKQCL AEFKRDLEWV ERLDVTLGPV PEIGGSEAPA PQNKDQKAVD PEDDFQREMS FYRQAQAAVL AVLPRLHQLK VPTKRPTDYF AEMAKSDLQM QKIRQKLQTK QAAMERSEKA KQLRALRKYG KKVQTEVLQK RQQEKAHMMN AIKKYQKGFS DKLDFLEGDQ KPLAQRKKAG AKGQQMRKGP SAKRRYKNQK FGFGGKKKGS KWNTRESYDD VSSFRAKTAH GRGLKRPGKK GSNKRPGKRT REKMKNRTH.

Product Science Overview

Gene and Protein Structure

The EBNA1BP2 gene is located on chromosome 1 and encodes a protein that is approximately 37.2 kDa in size . The protein consists of several domains that facilitate its interaction with other cellular components. Notably, EBNA1BP2 contains a conserved 200-300 amino acid block at its C-terminus, which is essential for its function .

Function and Biological Role

EBNA1BP2 is primarily involved in the processing of the 27S pre-rRNA, a critical step in the maturation of ribosomal RNA . This protein is necessary for the proper assembly of the ribosomal large subunit, which is essential for protein synthesis within the cell .

Interaction with Epstein-Barr Virus (EBV)

One of the most intriguing aspects of EBNA1BP2 is its interaction with the Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1). EBNA1 is a multifunctional DNA-binding protein that plays a key role in the replication and maintenance of the EBV episome within infected cells . The interaction between EBNA1 and EBNA1BP2 is crucial for the stable segregation of EBV episomes during cell division, ensuring the persistence of the virus in the host cell .

Recombinant EBNA1BP2

Recombinant EBNA1BP2 is typically produced in E. coli and is often tagged with a His-tag at the N-terminus to facilitate purification . This recombinant protein retains the functional properties of the native protein, making it a valuable tool for research into rRNA processing and EBV biology.

Applications in Research

Due to its role in rRNA processing and its interaction with EBV, EBNA1BP2 is a subject of interest in various research fields, including virology, molecular biology, and cancer research. Studies on EBNA1BP2 can provide insights into the mechanisms of ribosome biogenesis and the persistence of viral infections in host cells .

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