EBAG9 Human

Estrogen Receptor Binding Site Associated Antigen 9 Human Recombinant
Cat. No.
BT5738
Source
Escherichia Coli.
Synonyms
EB9, PDAF, RCAS1, EBAG9, Receptor-binding cancer antigen expressed on SiSo cells, Cancer-associated surface antigen RCAS1, Estrogen receptor-binding fragment-associated gene 9 protein.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

EBAG9 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 207 amino acids (28-213) and having a molecular mass of 23.4 kDa. The EBAG9 protein is fused to a 20 amino acid His-Tag at N-terminus and purified by standard chromatography techniques.

Product Specs

Introduction
EBAG9, a type III transmembrane protein found mainly in the Golgi apparatus, is considered a tumor-associated antigen. Its overexpression is observed in various cancers, including advanced breast and prostate cancers. While EBAG9 typically inhibits the growth of receptor-binding cells and promotes apoptosis in immune cells, cancer cells may utilize it to evade immune surveillance. Therefore, high EBAG9 expression can be linked to a negative prognosis. Research suggests EBAG9 contributes to bladder cancer progression. Conversely, in rheumatoid arthritis, EBAG9 deficiency may lead to CTL infiltration due to the inability to evade immune responses, causing apoptosis in synovial lining cells. EBAG9 is involved in regulating exocytosis and its expression plays a role in the progression of oral squamous cell carcinoma. Additionally, EBAG9 appears to have a specific function in the early stages of breast carcinogenesis.
Description
Recombinant human EBAG9, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 207 amino acids (residues 28-213). It has a molecular weight of 23.4 kDa. A 20 amino acid His-tag is fused to the N-terminus of the EBAG9 protein. Purification is achieved through standard chromatography techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The EBAG9 protein solution is supplied in a buffer containing 20mM Tris-HCl (pH 8), 1mM DTT, 2mM EDTA, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability, adding a carrier protein such as 0.1% HSA or BSA is advised. Importantly, repeated freezing and thawing of the product should be avoided.
Purity
The purity of this protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
EB9, PDAF, RCAS1, EBAG9, Receptor-binding cancer antigen expressed on SiSo cells, Cancer-associated surface antigen RCAS1, Estrogen receptor-binding fragment-associated gene 9 protein.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MRSGRGRKLS GDQITLPTTV DYSSVPKQTD VEEWTSWDED APTSVKIEGG NGNVATQQNS LEQLEPDYFK DMTPTIRKTQ KIVIKKREPL NFGIPDGSTG FSSRLAATQD LPFIHQSSEL GDLDTWQENT NAWEEEEDAA WQAEEVLRQQ KLADREKRAA EQQRKKMEKE AQRLMKKEQN KIGVKLS.

Product Science Overview

Gene and Protein Characteristics
  • Gene Name: EBAG9
  • Aliases: RCAS1, BAG9, PDAF
  • Protein Name: Estrogen receptor-binding fragment-associated gene 9 protein
  • UniProt ID: O00559
  • Entrez Gene ID: 9166
Function and Mechanism

EBAG9 is a tumor-associated antigen that is expressed at high frequency in various cancers, including advanced breast and prostate cancers . The protein plays a role in the regulation of cell growth and the apoptotic process (programmed cell death). It acts as a peptidase activator involved in the apoptotic process .

The regulation of transcription by estrogen is mediated by the estrogen receptor, which binds to the estrogen-responsive element found in the 5’-flanking region of the EBAG9 gene . This interaction is crucial for the gene’s expression and subsequent protein production.

Clinical Significance

EBAG9 is highly expressed in a variety of cancers, making it a significant marker for tumor progression and prognosis . Its expression is associated with immune evasion by cancer cells, as it can inhibit the growth of receptor-binding cells and induce apoptosis of immune cells . This makes EBAG9 a potential target for cancer immunotherapy.

Research and Applications

Human recombinant EBAG9 is used in various research applications, including:

  • Immunohistochemistry (IHC): Used to study the protein’s expression in different tissues, including cancerous tissues .
  • Immunofluorescence: Helps in visualizing the protein’s localization within cells .
  • ELISA: Used for detecting and quantifying the protein in various samples .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.