EAF1 Human

ELL Associated Factor 1 Human Recombinant

Cat. No.

BT5662

Source

Escherichia Coli.

Synonyms

ELL-associated factor 1, EAF1.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 95% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

EAF1 Human Recombinant produced in E. coli is. a single polypeptide chain containing 110 amino acids (182-268) and having a molecular mass of 11.3kDa. EAF1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

ELL Associated Factor 1 (EAF1), a member of the EAF family, is primarily found in the heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, and colon. It functions as a transcriptional transactivator, enhancing the elongation activities of ELL and ELL2.

Description

Recombinant human EAF1, produced in E. coli, is a single polypeptide chain consisting of 110 amino acids (residues 182-268). It has a molecular weight of 11.3 kDa. The protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.

Physical Appearance

A clear, sterile-filtered solution.

Formulation

The EAF1 solution (0.5 mg/ml) is formulated in 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, and 10% glycerol.

Stability

For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.

Purity

Purity exceeds 95% as determined by SDS-PAGE analysis.

Synonyms

ELL-associated factor 1, EAF1.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSDIIEQMS SSSGSSSSDS ESSSGSDDDS SSSGGEDNGP ASPPQPSHQQ PYNSRPAVAN GTSRPQGSNQ LMNTLRNDLQ LSESGSDSDD.

Product Science Overview

Gene and Protein Information

Gene Name: EAF1 (ELL Associated Factor 1)
Protein Name: EAF1 (Human Recombinant)
Gene ID: 85403
UniProtKB/Swiss-Prot ID: Q96JC9

Function

EAF1 is known to act as a transcriptional transactivator of ELL (Eleven-Nineteen Lysine-Rich Leukemia) and ELL2 elongation activities. It is involved in the regulation of transcription elongation from RNA polymerase II promoter. This protein is part of the transcription elongation factor complex and is located in the intercellular bridge and nuclear body.

Mechanism of Action

EAF1 enhances the self-association of ELL, which reduces its interaction with other components of the Super Elongation Complex (SEC). This interaction is crucial for the regulation of transcription during genotoxic stress. EAF1 also plays a role in the inhibition of HIV-1 transcription through the inhibition of super elongation complex formation.

Clinical Significance

Mutations or dysregulation of the EAF1 gene have been associated with various diseases, including microphthalmia, syndromic 10. The protein’s role in transcription elongation makes it a significant factor in understanding the mechanisms of gene expression and its implications in diseases.

Research and Applications

The recombinant form of EAF1 is used in various research applications to study its function and role in transcription regulation. It is also used to understand its interactions with other proteins and its impact on gene expression.

Pathways

EAF1 is involved in several pathways, including:

Formation of HIV elongation complex in the absence of HIV Tat
Gene expression (Transcription)

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EAF1 Human

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