DTYMK Human

The DTYMK gene is located on chromosome 2 and encodes a protein that consists of 232 amino acids . The enzyme has three important domains: the ligand-induced degradation (LID) domain, the nucleoside monophosphate (NMP) binding site, and the CORE domain . These domains are crucial for the enzyme’s function and stability.

DTYMK plays a pivotal role in the pyrimidine metabolism pathway, specifically in the synthesis of dTTP from dTMP . The enzyme’s activity is essential for DNA replication and repair, making it a critical component in cellular proliferation and maintenance . The reaction it catalyzes can be summarized as follows:

[ \text{ATP} + \text{dTMP} \rightarrow \text{ADP} + \text{dTDP} ]

Human recombinant DTYMK is typically produced in Escherichia coli (E. coli) expression systems. The recombinant protein is often tagged with a His-tag at the N-terminus to facilitate purification . The purified enzyme is used in various biochemical assays and research applications to study its function and role in DNA synthesis .

Mutations in the DTYMK gene have been associated with several diseases, including neurodegeneration with childhood-onset and progressive microcephaly . Additionally, the enzyme has been studied as a potential marker for certain types of cancer due to its role in cell proliferation .

Recombinant DTYMK is widely used in research to understand its role in DNA synthesis and its potential as a therapeutic target. Studies have explored its involvement in various cellular processes and its potential implications in disease mechanisms .