DTD1 Human

D-Tyrosyl-tRNA Deacylase 1 Human Recombinant
Cat. No.
BT23664
Source
Escherichia Coli.
Synonyms
bA379J5.3, bA555E18.1, C20orf88, DUEB, HARS2, pqn-68, D-tyrosyl-tRNA(Tyr) deacylase 1, DNA-unwinding element-binding protein B.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

DTD1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 232 amino acids (1-209a.a.) and having a molecular mass of 25.9kDa.
DTD1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
D-Tyrosyl-tRNA Deacylase 1 (DTD1), a member of the DTD family, plays a crucial role in hydrolyzing D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr), potentially acting as a defense mechanism against D-tyrosine's harmful effects. This ATPase is involved in DNA replication by facilitating CDC45 loading onto pre-replication complexes, specifically localizing to the DUE (DNA unwinding elements) of active replication origins. DTD1 is widely expressed in adult and fetal tissues, with the highest concentrations found in the testis, ovary, spleen, and brain. It may be a risk factor for AIA (aspirin-intolerant asthma) due to its ability to hydrolyze D-tryptophan and interact with tyrosyl-tRNA synthetase (tyrRS), which promotes a pro-inflammatory phenotype.
Description
Recombinant human DTD1, produced in E. coli, is a single, non-glycosylated polypeptide chain composed of 232 amino acids (1-209 a.a.) with a molecular weight of 25.9 kDa. The protein includes a 23 amino acid His-tag at the N-terminus and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The DTD1 protein solution (0.5 mg/ml) is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the protein can be stored at 4°C. For extended periods, it is recommended to store the protein frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the protein is determined to be greater than 85% by SDS-PAGE analysis.
Synonyms
bA379J5.3, bA555E18.1, C20orf88, DUEB, HARS2, pqn-68, D-tyrosyl-tRNA(Tyr) deacylase 1, DNA-unwinding element-binding protein B.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMKAVVQR VTRASVTVGG EQISAIGRGI CVLLGISLED TQKELEHMVR KILNLRVFED ESGKHWSKSVMDKQYEILCV SQFTLQCVLK GNKPDFHLAM PTEQAEGFYN SFLEQLRKTY RPELIKDGKF GAYMQVHIQN DGPVTIELES PAPGTATSDP KQLSKLEKQQ QRKEKTRAKG PSESSKERNT PRKEDRSASS GAEGDVSSER EP.

Product Science Overview

Function and Mechanism

DTD1 is an aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. It also deacylates mischarged glycyl-tRNA (Ala), protecting cells against glycine mischarging by AlaRS (Alanyl-tRNA synthetase). The enzyme acts via tRNA-based rather than protein-based catalysis, rejecting L-amino acids rather than detecting D-amino acids in the active site . By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, DTD1 counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality .

Genetic Information

The DTD1 gene is located on chromosome 20 and is also known by several aliases, including C20orf88 and HARS2. The gene is involved in coding for the DTD1 protein, which is essential for the hydrolysis of D-tyrosyl-tRNA (Tyr) into D-tyrosine and free tRNA (Tyr) . The encoded protein binds the DNA unwinding element and plays a role in the initiation of DNA replication .

Clinical Significance

Mutations or malfunctions in the DTD1 gene can lead to errors in protein synthesis, which may result in various cellular dysfunctions. The enzyme’s role in maintaining the accuracy of tRNA charging is vital for cellular health and function.

Research and Applications

Recombinant forms of D-Tyrosyl-tRNA Deacylase 1 are used in research to study its function and potential therapeutic applications. Understanding the enzyme’s mechanism can lead to insights into genetic disorders related to protein synthesis errors and the development of targeted treatments.

For more detailed information, you can refer to resources like GeneCards and UniProt .

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