DSTN Human

Destrin is a small, phosphoinositide-sensitive actin-binding protein. It is composed of 165 amino acids and has a molecular weight of approximately 19.5 kDa . The protein is capable of depolymerizing actin filaments in vitro, which is crucial for various cellular processes such as cell motility, division, and maintenance of cell shape .

Destrin is found in various epithelial and endothelial cells but is practically absent from adult mouse heart and skeletal muscle cells . It shares 71% sequence homology with cofilin, another member of the same superfamily, but the two proteins differ in their interaction with actin .

Recombinant human destrin is typically produced in E. coli and purified using conventional chromatography techniques . The recombinant protein often includes a C-terminal His-tag to facilitate purification and detection . The protein is stored in a buffer containing Tris-HCl, NaCl, glycerol, and DTT to maintain its stability .

Recombinant destrin is used in various research applications, including studies on actin dynamics, cell motility, and cytoskeletal organization. It is also used to investigate the molecular mechanisms underlying actin filament depolymerization and the role of actin-binding proteins in cellular processes .