DRG1 Human
Developmentally-regulated GTP-binding protein 1, DRG-1, Neural precursor cell expressed developmentally down-regulated protein 3, NEDD-3, DRG1, NEDD3.
Description
DRG1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 387 amino acids (1-367 a.a.) and having a molecular mass of 42.7kDa.
DRG1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Developmentally-regulated GTP-binding protein 1, DRG-1, Neural precursor cell expressed developmentally down-regulated protein 3, NEDD-3, DRG1, NEDD3.
MGSSHHHHHH SSGLVPRGSH MSSTLAKIAE IEAEMARTQK NKATAHHLGL LKARLAKLRR ELITPKGGGG GGPGEGFDVA KTGDARIGFV GFPSVGKSTL LSNLAGVYSE VAAYEFTTLT TVPGVIRYKG AKIQLLDLPG IIEGAKDGKG RGRQVIAVAR TCNLILIVLD VLKPLGHKKI IENELEGFGI RLNSKPPNIG FKKKDKGGIN LTATCPQSEL DAETVKSILA EYKIHNADVT LRSDATADDL IDVVEGNRVY IPCIYVLNKI DQISIEELDI IYKVPHCVPI SAHHRWNFDD LLEKIWDYLK LVRIYTKPKG QLPDYTSPVV LPYSRTTVED FCMKIHKNLI KEFKYALVWG LSVKHNPQKV GKDHTLEDED VIQIVKK.
Product Science Overview
Developmentally Regulated GTP Binding Protein 1 (DRG1) is a protein encoded by the DRG1 gene in humans. It is a member of the GTPase family, which are enzymes that hydrolyze guanosine triphosphate (GTP) to guanosine diphosphate (GDP). DRG1 is involved in various cellular processes, including microtubule dynamics, mitotic spindle assembly, and translation regulation.
The DRG1 gene is located on chromosome 22 and encodes a protein that contains structural motifs characteristic of GTP-binding proteins . The protein has intrinsic GTPase activity, which is stimulated by binding to ZC3H15/DFRP1, likely by increasing the affinity for potassium ions . The protein is composed of several domains, all of which are required for its microtubule-associated functions .
DRG1 catalyzes the conversion of GTP to GDP through the hydrolysis of the gamma-phosphate bond in GTP . This activity is essential for its role in promoting microtubule polymerization and stability, which are crucial for mitotic spindle assembly during cell division . Interestingly, the GTPase activity of DRG1 is not necessary for its microtubule-related functions .
In addition to its role in microtubule dynamics, DRG1 is involved in the regulation of translation. When hydroxylated at C-3 of ‘Lys-22’ by JMJD7, DRG1 may bind to RNA and play a role in translation . This suggests that DRG1 has a multifaceted role in cellular processes, extending beyond its GTPase activity.
DRG1 is expressed in various tissues and is developmentally regulated, meaning its expression levels change during different stages of development . It is involved in the positive regulation of microtubule polymerization and the regulation of mitotic spindle assembly, which are critical for proper cell division . Reduced levels of DRG1 in cells can lead to delayed progression from prophase to anaphase due to slowed spindle formation .
Mutations or dysregulation of the DRG1 gene have been associated with certain diseases. For example, DRG1 is linked to Tan-Almurshedi Syndrome and Intellectual Developmental Disorder, Autosomal Recessive 71 . Understanding the function and regulation of DRG1 can provide insights into the molecular mechanisms underlying these conditions and potentially lead to the development of therapeutic strategies.
