DPY30 Human

DPY30 Human Recombinant
Cat. No.
BT5255
Source
Escherichia Coli.
Synonyms
Dpy-30 Homolog (C. Elegans), Dpy-30-Like Protein, Dpy-30L, Protein Dpy-30 Homolog, HDPY-30, Cps25, Saf19, Protein dpy-30 homolog.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

DPY30 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 122 amino acids (1-99 a.a) and having a molecular mass of 13.6 kDa.
DPY30 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
DPY30 is involved in the trimethylation of histone H3 at the Lys-4 residue. This methylation is a marker for epigenetic activation of transcription. DPY30 is also involved in histone H3 acetylation. In teratocarcinoma cells, DPY30 plays a crucial role in retinoic acid-induced differentiation into neural lineages by regulating gene induction and H3 Lys-4 methylation at key developmental sites. Additionally, DPY30 is either directly or indirectly involved in endosomal transport.
Description
Recombinant human DPY30, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 122 amino acids (residues 1-99) and has a molecular weight of 13.6 kDa. The protein consists of a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The DPY30 protein solution is provided at a concentration of 1mg/ml. It is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 0.2M NaCl, 30% glycerol, and 2mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for prolonged storage. It is important to avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE.
Synonyms
Dpy-30 Homolog (C. Elegans), Dpy-30-Like Protein, Dpy-30L, Protein Dpy-30 Homolog, HDPY-30, Cps25, Saf19, Protein dpy-30 homolog.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMEPEQML EGQTQVAENP HSEYGLTDNV ERIVENEKIN AEKSSKQKVD LQSLPTRAYL DQTVVPILLQ GLAVLAKERP PNPIEFLASY LLKNKAQFED RN.

Product Science Overview

Introduction

DPY30 is a critical protein that plays a significant role in the regulation of gene expression through its involvement in histone modification. It is an integral core subunit of the SET1/MLL family of H3K4 methyltransferases, which are essential for the methylation of histone H3 at lysine 4 (H3K4). This methylation is a key epigenetic mark associated with gene activation .

Structure and Function

The DPY30 protein is composed of 99 amino acids and has a molecular weight of approximately 12 kDa . It is expressed in various tissues and is involved in the regulation of the cell cycle and differentiation of hematopoietic progenitor cells . DPY30 directly controls the expression of several key genes involved in these processes, making it crucial for maintaining the identity and function of adult hematopoietic stem cells (HSCs) .

Role in Hematopoiesis

DPY30 is particularly important in the hematopoietic system. Loss of DPY30 in adult hematopoietic cells leads to severe pancytopenia, a condition characterized by a reduction in the number of red and white blood cells, as well as platelets . This loss also results in the accumulation of HSCs and early hematopoietic progenitor cells (HPCs) that are defective in multilineage reconstitution, indicating a block in differentiation . DPY30 facilitates genome-wide H3K4 methylation, which is necessary for the proper expression of genes involved in hematopoietic development .

Recombinant DPY30

Recombinant human DPY30 is produced using an E. coli expression system and is typically tagged with an N-terminal His tag for purification purposes . The recombinant protein is used in various research applications, including studies on gene regulation, epigenetics, and hematopoiesis. It is supplied in a sterile buffer and should be stored at -70°C to maintain its stability and activity .

Applications in Research

The recombinant DPY30 protein is valuable for studying the mechanisms of histone methylation and its impact on gene expression. It is also used to investigate the role of epigenetic modifications in the regulation of stem cell differentiation and the development of hematopoietic diseases . By understanding the function of DPY30, researchers can gain insights into the epigenetic regulation of gene expression and develop potential therapeutic strategies for diseases associated with dysregulated histone methylation.

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