DNTTIP1 Human

Deoxynucleotidyltransferase Terminal Interacting Protein 1 Human Recombinant
Cat. No.
BT4876
Source
Escherichia Coli.
Synonyms
Deoxynucleotidyltransferase Terminal Interacting Protein 1, Novel Protein Similar To Synaptotagmin 1, Terminal Deoxynucleotidyltransferase-Interacting Factor 1, Chromosome 20 Open Reading Frame 167, Tdif1, C20orf167, TdT-Interacting Factor 1, TdT Binding Protein, dJ447F3.4, TDIF1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

DNTTIP1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 352 amino acids (1-329) and having a molecular mass of 39.0kDa.
DNTTIP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The protein DNTTIP1 interacts with DNA molecules and enhances the catalytic activity of terminal deoxynucleotidyltransferase (also known as TDT or DNTT). TDT is a DNA polymerase enzyme that facilitates the polymerization of DNA without the need for a template strand.
Description
Recombinant human DNTTIP1, expressed in E. coli, is a single polypeptide chain that lacks glycosylation. It comprises 352 amino acids, with residues 1-329 representing the DNTTIP1 sequence, and has a molecular weight of 39.0 kDa. The protein includes a 23-amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear solution, devoid of particulate matter, that has undergone sterile filtration.
Formulation
The DNTTIP1 solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 1 mM DTT, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the solution should be maintained at a temperature of 4 degrees Celsius. For extended storage, the solution should be stored frozen at -20 degrees Celsius. The addition of a carrier protein, such as 0.1% HSA or BSA, is recommended for long-term storage. Repeated cycles of freezing and thawing should be avoided.
Purity
The purity of the DNTTIP1 protein is greater than 85.0%, as assessed by SDS-PAGE analysis.
Synonyms
Deoxynucleotidyltransferase Terminal Interacting Protein 1, Novel Protein Similar To Synaptotagmin 1, Terminal Deoxynucleotidyltransferase-Interacting Factor 1, Chromosome 20 Open Reading Frame 167, Tdif1, C20orf167, TdT-Interacting Factor 1, TdT Binding Protein, dJ447F3.4, TDIF1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGATGDA EQPRGPSGAE RGGLELGDAG AAGQLVLTNP WNIMIKHRQV QRRGRRSQMT TSFTDPAISM DLLRAVLQPS INEEIQTVFN KYMKFFQKAA LNVRDNVGEE VDAEQLIQEA CRSCLEQAKL LFSDGEKVIP RLTHELPGIK RGRQAEEECA HRGSPLPKKR KGRPPGHILS SDRAAAGMVW KPKSCEPIRR EGPKWDPARL NESTTFVLGS RANKALGMGG TRGRIYIKHP HLFKYAADPQ DKHWLAEQHH MRATGGKMAY LLIEEDIRDL AASDDYRGCL DLKLEELKSF VLPSWMVEKM RKYMETLRTE NEHRAVEAPP QT

Product Science Overview

Structure and Function

DNTTIP1 binds to DNA and increases the activity of TdT. This interaction is significant because TdT adds nucleotides to the V, D, and J exons of T-cell receptor (TCR) and B-cell receptor (BCR) genes during the process of antibody gene recombination. This addition of nucleotides contributes to the phenomenon known as junctional diversity, which is essential for the diversity of the immune response .

In addition to its role in enhancing TdT activity, DNTTIP1 also acts as a transcriptional regulator. It binds to specific DNA sequences and can associate with promoters, such as the RAB20 promoter, to positively regulate transcription. DNTTIP1 has been shown to bind to nucleosomes and may recruit histone deacetylase 1 (HDAC1) complexes to nucleosomes or naked DNA, influencing chromatin structure and gene expression .

Biological Significance

The activity of DNTTIP1 and its interaction with TdT are critical for the proper functioning of the immune system. The diversity introduced by TdT during the recombination of TCR and BCR genes allows for a wide variety of antigen receptors, which are necessary for the immune system to recognize and respond to a vast array of pathogens. Studies using TdT knockout mice have demonstrated a significant reduction in TCR diversity, highlighting the importance of TdT and its interacting proteins like DNTTIP1 in immune function .

Clinical Relevance

Mutations or dysregulation of the DNTTIP1 gene have been associated with certain diseases. For example, DNTTIP1 has been linked to nephrotic syndrome, a kidney disorder characterized by excessive protein loss in the urine. Understanding the role of DNTTIP1 in such diseases could provide insights into potential therapeutic targets .

Research and Applications

Recombinant human DNTTIP1 is used in various research applications to study its function and interactions. By using recombinant proteins, researchers can investigate the biochemical properties of DNTTIP1, its role in enhancing TdT activity, and its involvement in transcriptional regulation. These studies contribute to a deeper understanding of the molecular mechanisms underlying immune diversity and gene regulation .

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