DNMT3L Human

DNA Cytosine-5--Methyltransferase 3-Like Human Recombinant
Cat. No.
BT3751
Source
Escherichia Coli.
Synonyms
DNMT3L, DNA (Cytosine-5-)-Methyltransferase 3-Like, Human Cytosine-5-Methyltransferase 3-Like Protein 11, Cytosine-5-Methyltransferase 3-Like Protein, DNA (Cytosine-5)-Methyltransferase 3-Like.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

DNMT3L Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 411 amino acids (1-386) and having a molecular mass of 46.2kDa.
DNMT3L is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is a protein found in the cell nucleus that resembles DNA methyltransferases. However, it is not thought to directly add methyl groups to DNA because it lacks the necessary amino acid building blocks for this activity. Despite this, DNMT3L enhances the addition of methyl groups to DNA by another enzyme called DNA cytosine methyltransferase 3 alpha. This interaction is considered crucial for establishing specific patterns of DNA methylation inherited from the mother. DNMT3L is also involved in suppressing gene activity by interacting with histone deacetylase 1. In essence, DNMT3L is a regulatory factor that, while inactive in directly modifying DNA methylation, plays a vital role in controlling the activity of DNMT3A and DNMT3B, two enzymes that establish DNA methylation patterns.
Description
This product consists of the human DNMT3L protein manufactured in E. coli bacteria. It is a single chain of amino acids that is not glycosylated, meaning it does not have sugars attached. The protein comprises 411 amino acids (specifically, amino acids 1 to 386 of the full-length protein) and has a molecular weight of 46.2 kDa. For purification and ease of use, a 25 amino acid His-tag is attached to the start of the protein. The purification process utilizes specialized chromatographic methods.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The DNMT3L protein is provided at a concentration of 0.25 mg/ml in a solution containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the DNMT3L protein is greater than 90%, as assessed by SDS-PAGE.
Synonyms
DNMT3L, DNA (Cytosine-5-)-Methyltransferase 3-Like, Human Cytosine-5-Methyltransferase 3-Like Protein 11, Cytosine-5-Methyltransferase 3-Like Protein, DNA (Cytosine-5)-Methyltransferase 3-Like.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSEFMAAIP ALDPEAEPSM DVILVGSSEL SSSVSPGTGR DLIAYEVKAN QRNIEDICIC CGSLQVHTQH PLFEGGICAP CKDKFLDALF LYDDDGYQSY CSICCSGETL LICGNPDCTR CYCFECVDSL VGPGTSGKVH AMSNWVCYLC LPSSRSGLLQ RRRKWRSQLK AFYDRESENP LEMFETVPVW RRQPVRVLSL FEDIKKELTS LGFLESGSDP GQLKHVVDVT DTVRKDVEEW GPFDLVYGAT PPLGHTCDRP PSWYLFQFHR LLQYARPKPG SPRPFFWMFV DNLVLNKEDL DVASRFLEME PVTIPDVHGG SLQNAVRVWS NIPAIRSRHW ALVSEEELSL LAQNKQSSKL AAKWPTKLVK NCFLPLREYF KYFSTELTSS L.

Product Science Overview

Function and Mechanism

DNMT3L is part of the DNA methyltransferase family, which includes DNMT1, DNMT3A, and DNMT3B. Unlike DNMT3A and DNMT3B, which are responsible for de novo DNA methylation, DNMT3L itself lacks catalytic activity. Instead, it acts as a regulatory factor that enhances the activity of DNMT3A and DNMT3B by forming complexes with them . This interaction is essential for the establishment of DNA methylation patterns during embryonic development, genomic imprinting, and X-chromosome inactivation .

Biological Importance

DNA methylation is a critical epigenetic modification that influences various biological processes, including:

  • Embryonic Development: Proper DNA methylation is essential for normal development. DNMT3L, by regulating DNMT3A and DNMT3B, ensures that methylation patterns are correctly established in the early stages of development .
  • Genomic Imprinting: This process involves the selective expression of genes based on their parental origin. DNMT3L plays a role in establishing the methylation marks that are necessary for imprinting .
  • X-Chromosome Inactivation: In females, one of the X chromosomes is inactivated to achieve dosage compensation. DNMT3L contributes to the methylation processes that silence one of the X chromosomes .
Research and Applications

The study of DNMT3L has significant implications in understanding epigenetic regulation and its impact on health and disease. Abnormal DNA methylation patterns are associated with various diseases, including cancer, neurological disorders, and developmental abnormalities . Research on DNMT3L and its interactions with other DNA methyltransferases can provide insights into the mechanisms underlying these conditions and potentially lead to therapeutic interventions.

In the context of recombinant protein production, human DNMT3L can be expressed and purified for use in biochemical assays and structural studies. This recombinant form allows researchers to investigate the enzyme’s properties and interactions in a controlled environment, facilitating the development of targeted therapies and diagnostic tools .

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