DNAL1 Human
Dynein Axonemal Light Chain 1 Human Recombinant
Cat. No.
BT215
Source
E.coli.
Synonyms
Dynein light chain 1, axonemal, C14orf168, CILD16, DNAL1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
DNAL1 Human Recombinant produced in E. coli is a single polypeptide chain containing 213 amino acids (1-190) and having a molecular mass of 23.9 kDa. DNAL1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
The protein known as Dynein Axonemal Light Chain 1 (DNAL1) is a part of the outer dynein arms complex. This complex plays a crucial role in the movement of cilia, which are hair-like structures found on the surface of many cells. DNAL1 acts as a molecular motor, using energy from ATP to generate the force required for ciliary movement. It is primarily found in tissues that have motile cilia or flagella, such as those involved in sperm motility. Interestingly, there are multiple forms of the DNAL1 protein, known as transcript variants, which are generated through a process called alternative splicing.
Description
This product consists of the human DNAL1 protein, which has been recombinantly produced in E. coli bacteria. The protein is a single chain of 213 amino acids (specifically amino acids 1-190 of the full-length protein), resulting in a molecular weight of 23.9 kDa. To facilitate purification and detection, the DNAL1 protein has been engineered with a 23 amino acid His-tag at its N-terminus. The protein has been purified using proprietary chromatographic techniques to ensure its high quality and purity.
Physical Appearance
The product is a sterile and colorless solution that has been filtered for clarity.
Formulation
The DNAL1 protein is supplied in a solution containing 1mg/ml of protein in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.4M Urea, and 10% glycerol.
Stability
To ensure product stability, it is recommended to store the solution at 4°C if the entire vial will be used within 2-4 weeks. For longer-term storage, it is advisable to store the solution frozen at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein such as HSA or BSA (0.1%). It is important to avoid repeated freeze-thaw cycles to prevent protein degradation.
Purity
The purity of the DNAL1 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Dynein light chain 1, axonemal, C14orf168, CILD16, DNAL1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAKATTI KEALARWEEK TGQRPSEAKE IKLYAQIPPI EKMDASLSML ANCEKLSLST NCIEKIANLN GLKNLRILSL GRNNIKNLNG LEAVGDTLEE LWISYNFIEK LKGIHIMKKL KILYMSNNLV KDWAEFVKLA ELPCLEDLVF VGNPLEEKHS AENNWIEEAT KRVPKLKKLD GTPVIKGDEE EDN.
Product Science Overview
Function
Clinical Significance
Mutations in the DNAL1 gene are associated with Primary Ciliary Dyskinesia (PCD), a rare genetic disorder characterized by dysfunctional and structurally abnormal cilia . PCD affects the respiratory system, leading to repeated respiratory infections, pneumonia, and even infertility . In severe cases, patients with PCD may develop respiratory failure .
Research and Applications
Recombinant human LC1 is used in research to study the structure and function of cilia, as well as to understand the molecular mechanisms underlying PCD . Accurate quantitation of LC1 in tissues, such as lung tissue, is crucial for developing potential therapies for PCD . Advanced techniques like immunoprecipitation combined with nanoLC-MS/MS are employed to measure the expression levels of LC1 in complex biological matrices .
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
