DnaK E.coli
Greater than 85.0% as determined by SDS-PAGE.
Description
Product Specs
Product Science Overview
DnaK, also known as HSP70, is a highly conserved molecular chaperone found in Escherichia coli (E. coli). It plays a crucial role in protein homeostasis by assisting in the folding of newly synthesized polypeptides, preventing the aggregation of misfolded proteins, and aiding in the refolding of stress-denatured proteins .
DnaK is a member of the HSP70 family of heat shock proteins, which are characterized by their ability to bind and hydrolyze ATP. The protein consists of three main domains:
- N-terminal ATPase domain: Responsible for ATP binding and hydrolysis.
- Substrate-binding domain: Binds to exposed hydrophobic regions of unfolded or partially folded proteins.
- C-terminal domain: Plays a role in the regulation of the chaperone activity.
The ATPase activity of DnaK is essential for its function as a chaperone. The binding and hydrolysis of ATP induce conformational changes in DnaK, allowing it to interact with substrate proteins and facilitate their proper folding .
In E. coli, DnaK is involved in various cellular processes, including:
- Protein Folding: DnaK assists in the folding of newly synthesized proteins and prevents the aggregation of misfolded proteins.
- Stress Response: During stress conditions, such as heat shock, DnaK helps in the refolding of denatured proteins and protects cells from stress-induced damage .
- Regulation of Gene Expression: DnaK plays a role in regulating the activity of the heat shock sigma factor σ32, which controls the expression of heat shock proteins .
Recombinant DnaK is produced by cloning the dnaK gene from E. coli into an expression vector and expressing the protein in a suitable host system. This allows for the production of large quantities of DnaK for research and industrial applications. Recombinant DnaK retains the functional properties of the native protein and is used in studies related to protein folding, stress response, and chaperone activity .
Recombinant DnaK has several applications in research and biotechnology:
- Protein Folding Studies: Used to study the mechanisms of protein folding and the role of chaperones in this process.
- Stress Response Research: Helps in understanding the cellular response to stress and the role of chaperones in protecting cells from damage.
- Biotechnological Applications: Used in the production of recombinant proteins to improve their yield and stability by preventing aggregation and misfolding.
