DNAJC27 Human

DnaJ (Hsp40) Homolog, Subfamily C, Member 27 Human Recombinant
Cat. No.
BT15196
Source
E.coli.
Synonyms
DnaJ homolog subfamily C member 27, DnaJ (Hsp40) Homolog, Subfamily C, Member 27, DNAJC27, Rab and DnaJ domain-containing protein, RABJS, RBJ, dnaJ homolog subfamily C member 27 isoform 1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

DNAJC27 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 296 amino acids (1-273 a.a.) and having a molecular mass of 33.2kDa.
DNAJC27 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
DNAJC27, also known as DnaJ (Hsp40) Homolog, Subfamily C, Member 27, is a protein that in humans is encoded by the DNAJC27 gene. This protein is a member of the heat shock protein 40 (Hsp40) family and contains a conserved J domain. DNAJC27 functions as a GTPase, which means it plays a role in regulating cellular processes by binding to and hydrolyzing GTP. Specifically, DNAJC27 has been shown to activate the MEK/ERK signaling pathway, which is involved in cell growth, proliferation, and survival. Overexpression of DNAJC27 can lead to cell transformation, suggesting a potential role in cancer development.
Description
This recombinant DNAJC27 protein is produced in E. coli bacteria and corresponds to amino acids 1-273 of the human protein sequence. It is a single, non-glycosylated polypeptide chain with a molecular weight of 33.2 kDa. For purification purposes, a 23 amino acid His-tag is fused to the N-terminus of the protein. Purification is achieved using proprietary chromatographic techniques. The resulting protein is greater than 90% pure.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The DNAJC27 protein solution is supplied at a concentration of 0.25 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 50% glycerol, 2 mM DTT, and 2 mM EDTA.
Stability
For short-term storage (2-4 weeks), the DNAJC27 solution can be stored at 4°C. For long-term storage, it is recommended to store the solution frozen at -20°C. Adding a carrier protein such as HSA or BSA to a final concentration of 0.1% is advisable for long-term storage. To maintain protein stability, avoid repeated freeze-thaw cycles.
Purity
The purity of the DNAJC27 protein is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
DnaJ homolog subfamily C member 27, DnaJ (Hsp40) Homolog, Subfamily C, Member 27, DNAJC27, Rab and DnaJ domain-containing protein, RABJS, RBJ, dnaJ homolog subfamily C member 27 isoform 1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMEANMPK RKEPGRSLRI KVISMGNAEV GKSCIIKRYC EKRFVSKYLA TIGIDYGVTK VHVRDREIKV NIFDMAGHPF FYEVRNEFYK DTQGVILVYD VGQKDSFDAL DAWLAEMKQE LGPHGNMENI IFVVCANKID CTKHRCVDES EGRLWAESKG FLYFETSAQT GEGINEMFQT FYISIVDLCE NGGKRPTTNS SASFTKEQAD AIRRIRNSKD SWDMLGVKPG ASRDEVNKAY RKLAVLLHPD KCVAPGSEDA FKAVVNARTA LLKNIK.

Product Science Overview

Introduction

DnaJ (Hsp40) Homolog, Subfamily C, Member 27, also known as DNAJC27, is a member of the DnaJ heat shock protein family (Hsp40). This family of proteins plays a crucial role in the cellular response to stress by acting as molecular chaperones, which assist in the proper folding and functioning of other proteins.

Gene and Protein Structure

The DNAJC27 gene is located on chromosome 2p23.3 . The gene encodes a protein that consists of 273 amino acids and contains a RAB-like GTPase domain followed by a DNAJ domain . There is also a splice variant of this gene that encodes a protein with only the GTPase domain .

Function

DNAJC27 is predicted to have GTPase activity, which is essential for intracellular protein transport and the positive regulation of the MAPK cascade . The protein can activate the MEK/ERK pathway and induce cell transformation when overexpressed . It may also act as a nuclear scaffold for MAPK1, enhancing ERK1/ERK2 signaling .

Expression

The expression of DNAJC27 is highly prevalent in the nervous system and reproductive organs . This widespread expression suggests that DNAJC27 plays a significant role in various physiological processes.

Associated Diseases

Mutations or dysregulation of the DNAJC27 gene have been associated with several disorders, including labyrinthine bilateral reactive loss and separation anxiety disorder . These associations highlight the importance of DNAJC27 in maintaining normal cellular functions and its potential involvement in disease mechanisms.

Research and Applications

Research on DNAJC27 continues to uncover its various roles in cellular processes and its potential as a therapeutic target. The recombinant form of this protein is used in various studies to understand its function and to develop potential treatments for diseases associated with its dysregulation.

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