DNAJC24 Human

DnaJ (Hsp40) Homolog, Subfamily C, Member 24 Human Recombinant
Cat. No.
BT15133
Source
E.coli.
Synonyms
DnaJ homolog subfamily C member 24, DNAJC24, DPH4, JJJ3, ZCSL3, CSL-type zinc finger-containing protein 3, Diphthamide biosynthesis protein 4.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

DNAJC24 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 172 amino acids (1-149 a.a.) and having a molecular mass of 19.5kDa. DNAJC24 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
As a member of the ZCSL protein family, DnaJ homolog subfamily C member 24 (DNAJC24) possesses a DPH-type zinc finger. Its role involves the synthesis of diphthamide, a crucial component of translation elongation factor EF-2, which is targeted by bacterial ADP-ribosylating toxins. DNAJC24 also stimulates the ATPase activity of certain Hsp70-type chaperones and is found in various tissues including the heart, brain, spleen, lung, liver, kidney, and testis.
Description
Recombinant DNAJC24 Human, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 172 amino acids (1-149 a.a.) with a molecular weight of 19.5 kDa. This protein is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The DNAJC24 solution (1mg/ml) is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 10% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), keep refrigerated at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
DnaJ homolog subfamily C member 24, DNAJC24, DPH4, JJJ3, ZCSL3, CSL-type zinc finger-containing protein 3, Diphthamide biosynthesis protein 4.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMMAVEQM PKKDWYSILG ADPSANISDL KQKYQKLILM YHPDKQSTDV PAGTVEECVQ KFIEIDQAWK ILGNEETKRE YDLQRCEDDL RNVGPVDAQV YLEEMSWNEG DHSFYLSCRC GGKYSVSKDE AEEVSLISCD TCSLIIELLH YN.

Product Science Overview

Introduction

DnaJ (Hsp40) Homolog, Subfamily C, Member 24, also known as DNAJC24, is a member of the DnaJ/Hsp40 family of proteins. This family is known for its role in protein folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of Hsp70-type chaperones . DNAJC24 is a protein-coding gene that plays a crucial role in various cellular processes.

Gene and Protein Structure

The DNAJC24 gene is located on chromosome 11 and encodes a protein that contains a highly conserved J domain, which is characteristic of the DnaJ/Hsp40 family . This domain is essential for binding to Hsp70 chaperones and enhancing their ATPase activity. The protein also has iron-binding capabilities, which enhance its function as an electron carrier .

Function and Mechanism

DNAJC24 is involved in the biosynthesis of diphthamide, a unique post-translationally modified histidine found in translation elongation factor-2 (EEF2) . This modification is conserved across species and serves as a target for ADP-ribosylation and inactivation by diphtheria toxin and Pseudomonas exotoxin A . The iron-bound form of DNAJC24 is redox-active and can function as an electron carrier, further enhancing its role in cellular processes .

Biological Pathways

DNAJC24 is associated with several biological pathways, including the metabolism of proteins and the gamma carboxylation, hypusinylation, hydroxylation, and arylsulfatase activation pathways . These pathways are crucial for maintaining cellular homeostasis and proper protein function.

Clinical Significance

Mutations or dysregulation of DNAJC24 have been linked to various diseases, including diphtheria and Joubert Syndrome 4 . Understanding the function and regulation of DNAJC24 can provide insights into the mechanisms underlying these diseases and potentially lead to the development of targeted therapies.

Recombinant Form

The recombinant form of DNAJC24 is produced using recombinant DNA technology, which involves inserting the DNAJC24 gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant protein can be used for various research and therapeutic purposes, including studying its function, interactions, and potential as a drug target.

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