DNAJB4 Human
DnaJ (Hsp40) Homolog, Subfamily B, Member 4 Human Recombinant
Cat. No.
BT14769
Source
Escherichia Coli.
Synonyms
DnaJ homolog subfamily B member 4, Heat shock 40 kDa protein 1 homolog, HSP40 homolog, Heat shock protein 40 homolog, Human liver DnaJ-like protein, DNAJB4, DNAJW, HLJ1, DjB4.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
DNAJB4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 360 amino acids (1-337 a.a.) and having a molecular mass of 40kDa.
DNAJB4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
DNAJB4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
DNAJB4 (DnaJ (Hsp40) Homolog, Subfamily B, Member 4) is a protein belonging to the DNAJ/HSP40 protein family, known for its evolutionary conservation. It typically exists as a homodimer and is believed to function as a chaperone. DNAJB4 interacts with several other proteins, including OPRM1 through its C-terminal section and SDIM1. Its expression is highest in the heart, pancreas, and skeletal muscle, with lower levels detected in the brain, placenta, and liver.
Description
This product consists of a recombinant human DNAJB4 protein produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 360 amino acids (amino acids 1-337), resulting in a molecular weight of 40 kDa. The protein includes a 23 amino acid His-tag attached to the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The DNAJB4 protein is supplied in a solution at a concentration of 0.25 mg/ml. The solution also contains 20mM Tris-HCl buffer (pH 7.5), 0.2M NaCl, 30% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein like HSA or BSA to a final concentration of 0.1% is advisable for long-term storage. Repeated freezing and thawing of the product should be minimized.
Purity
Purity is confirmed to be greater than 90% using SDS-PAGE analysis.
Synonyms
DnaJ homolog subfamily B member 4, Heat shock 40 kDa protein 1 homolog, HSP40 homolog, Heat shock protein 40 homolog, Human liver DnaJ-like protein, DNAJB4, DNAJW, HLJ1, DjB4.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGKDYYC ILGIEKGASD EDIKKAYRKQ ALKFHPDKNK SPQAEEKFKE VAEAYEVLSD PKKREIYDQF GEEGLKGGAG GTDGQGGTFR YTFHGDPHAT FAAFFGGSNP FEIFFGRRMG GGRDSEEMEI DGDPFSAFGF SMNGYPRDRN SVGPSRLKQD PPVIHELRVS LEEIYSGCTK RMKISRKRLN ADGRSYRSED KILTIEIKKG WKEGTKITFP REGDETPNSI PADIVFIIKD KDHPKFKRDG SNIIYTAKIS LREALCGCSI NVPTLDGRNI PMSVNDIVKP GMRRRIIGYG LPFPKNPDQR GDLLIEFEVS FPDTISSSSK EVLRKHLPAS.
Product Science Overview
Structure and Function
DNAJB4 is a molecular chaperone that assists in the proper folding of proteins and the degradation of misfolded proteins. It is known to bind to the cell adhesion protein E-cadherin, targeting it to the plasma membrane. Additionally, DNAJB4 binds incorrectly folded E-cadherin and directs it for endoplasmic reticulum-associated degradation .
The protein is involved in several cellular processes, including:
- Protein folding: DNAJB4 stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B .
- Tumor suppression: DNAJB4 acts as a tumor suppressor, particularly in colorectal carcinoma. Downregulation of this gene may serve as a biomarker for predicting patient outcomes .
- Cellular stress response: As a member of the heat shock protein family, DNAJB4 helps cells cope with stress by preventing the aggregation of misfolded proteins .
Genetic Information
Clinical Significance
Mutations or alterations in the DNAJB4 gene have been associated with various diseases, including:
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