DnaJ Antibody

DnaJ (HSP40), Mouse Anti Human
Cat. No.
BT10797
Source
Synonyms
HSP-40, HSP40, DnaJ, DNAJB1, HSPF1, Hdj1, DnaJ homolog subfamily B member 1, Heat shock 40 kDa protein 1, Heat shock protein 40, DnaJ protein homolog 1, HDJ-1, Sis1.
Appearance
Sterile Filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Human HSP40, a heat shock protein with a molecular weight of 40kDa, is a mammalian homolog of the bacterial DnaJ heat shock protein. It plays a crucial role in regulating cellular processes by assisting in protein folding, transport, and assembly. HSP40 contains a J-domain, which is responsible for interacting with the ATPase domain of DnaK.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The antibody is supplied at a concentration of 1mg/ml in a solution of phosphate-buffered saline (PBS) at pH 7.4 with 0.1% sodium azide added as a preservative.
Storage Procedures
For short-term storage of up to 1 month, the antibody should be kept at 4°C. For long-term storage, it is recommended to store the antibody at -20°C. To maintain the stability and activity of the antibody, it is crucial to avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody has a shelf life of 12 months when stored properly at -20°C. If stored at 4°C, the antibody is stable for 1 month.
Applications
This DnaJ antibody has been rigorously tested using ELISA and Western blot analysis to ensure its specificity and reactivity. However, as applications can vary, it is recommended to perform a titration for each experiment to optimize results. For Western blot analysis, the recommended dilution range is 1:1,000 to 1:2,000, with a starting dilution of 1:1,000.
Synonyms
HSP-40, HSP40, DnaJ, DNAJB1, HSPF1, Hdj1, DnaJ homolog subfamily B member 1, Heat shock 40 kDa protein 1, Heat shock protein 40, DnaJ protein homolog 1, HDJ-1, Sis1.
Purification Method
DnaJ antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
Pk1C7AT.
Immunogen
Anti-human DnaJ mAb, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human DnaJ amino acids 1-340 purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and κ light chain.

Product Science Overview

Introduction

DnaJ (HSP40) proteins are a family of molecular chaperones that play a crucial role in protein folding, repair, and degradation. These proteins are essential for maintaining cellular homeostasis, especially under stress conditions. The term “Mouse Anti Human” refers to antibodies produced in mice that are specific to human proteins. In this context, the focus is on the DnaJ (HSP40) protein and its significance in human biology.

Structure and Function of DnaJ (HSP40)

DnaJ proteins, also known as HSP40 (Heat Shock Protein 40), are characterized by the presence of a J-domain, which is essential for their interaction with HSP70 proteins. The J-domain facilitates the binding of substrate proteins to HSP70, thereby assisting in their proper folding and preventing aggregation . DnaJ proteins are divided into three main classes based on their domain structure:

  • Class I: Contains a J-domain, a Gly/Phe-rich region, and a zinc finger-like domain.
  • Class II: Lacks the zinc finger-like domain but retains the J-domain and Gly/Phe-rich region.
  • Class III: Contains only the J-domain.
Role in Cellular Processes

DnaJ (HSP40) proteins are involved in various cellular processes, including:

  • Protein Folding: They assist HSP70 in the proper folding of nascent and misfolded proteins.
  • Protein Degradation: They target damaged or misfolded proteins for degradation via the ubiquitin-proteasome system.
  • Stress Response: They are upregulated in response to cellular stress, such as heat shock, to protect cells from damage .
Mouse Anti Human DnaJ (HSP40) Antibodies

Mouse Anti Human antibodies are monoclonal or polyclonal antibodies produced in mice that specifically recognize human DnaJ (HSP40) proteins. These antibodies are widely used in research and diagnostic applications, including:

  • Western Blotting: To detect the presence and quantity of DnaJ (HSP40) proteins in various samples.
  • Immunofluorescence: To visualize the localization of DnaJ (HSP40) proteins within cells.
  • Immunoprecipitation: To isolate DnaJ (HSP40) proteins from complex mixtures for further analysis .
Applications in Research

DnaJ (HSP40) proteins and their corresponding antibodies are valuable tools in biomedical research. They are used to study:

  • Neurodegenerative Diseases: DnaJ proteins are implicated in the prevention of protein aggregation, a hallmark of diseases such as Alzheimer’s and Parkinson’s .
  • Cancer: Altered expression of DnaJ proteins has been observed in various cancers, making them potential targets for therapeutic intervention.
  • Infectious Diseases: DnaJ proteins play a role in the immune response to infections, and their antibodies can be used to study pathogen-host interactions .

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