DIRAS1 Human

DIRAS family, GTP-binding RAS-like 1 Human Recombinant
Cat. No.
BT6723
Source
Escherichia Coli.
Synonyms
GTP-binding protein Di-Ras1, Distinct subgroup of the Ras family member 1, Ras-related inhibitor of cell growth, Rig, Small GTP-binding tumor suppressor 1, DIRAS1, GBTS1, Di-Ras1, FLJ42681.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

DIRAS1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 215 amino acids (1-195a.a.) and having a molecular mass of 24.1 kDa. DIRAS1 protein is fused to a 20 amino acid His tag at N-terminus and is purified by standard chromatography.

Product Specs

Introduction
DIRAS1, a member of the Ras superfamily of GTPases, plays a crucial role in cellular signaling. It exhibits low GTPase activity and primarily exists in the GTP-bound state. DIRAS1, like other Ras proteins, functions as a molecular switch, regulating pathways involved in various cellular processes, including cell growth, differentiation, adhesion, migration, and apoptosis. Dysregulation of DIRAS1 and other Ras-related proteins is implicated in cancer development and progression, contributing to increased invasion, metastasis, and reduced cell death.
Description
Recombinant human DIRAS1 protein, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 215 amino acids (residues 1-195). It has a molecular weight of 24.1 kDa. The protein is fused to a 20-amino acid Histidine tag at the N-terminus to facilitate purification using standard chromatography techniques.
Physical Appearance
Clear, colorless solution free from particulate matter.
Formulation
The DIRAS1 human solution is provided at a concentration of 1 mg/ml in a buffer containing 20mM Tris-HCl (pH 8.0), 20% glycerol, 1mM DTT, 0.1M NaCl, and 1mM EDTA.
Stability
For short-term storage (up to 4 weeks), keep the solution refrigerated at 4°C. For extended periods, store the protein at -20°C. To ensure long-term stability, adding a carrier protein such as HSA or BSA (0.1%) is recommended. Repeated freezing and thawing should be avoided.
Purity
The purity of the DIRAS1 protein is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
GTP-binding protein Di-Ras1, Distinct subgroup of the Ras family member 1, Ras-related inhibitor of cell growth, Rig, Small GTP-binding tumor suppressor 1, DIRAS1, GBTS1, Di-Ras1, FLJ42681.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MPEQSNDYRV VVFGAGGVGK SSLVLRFVKG TFRDTYIPTI EDTYRQVISC DKSVCTLQIT DTTGSHQFPA MQRLSISKGH AFILVFSVTS KQSLEELGPI YKLIVQIKGS VEDIPVMLVG KCDETQREV DTREAQAVAQ EWKCAFMETS AKMNYNVKEL FQELLTLETR RNMSLNIDGK RSGKQKRTDR KGKC.

Product Science Overview

Introduction

DIRAS family, GTP-binding RAS-like 1 (DIRAS1), also known as Di-Ras1, is a member of the Ras superfamily of monomeric GTPases. This protein is encoded by the DIRAS1 gene in humans and is located on chromosome 19 (19p13.3) . DIRAS1 is known for its low GTPase activity and its predominant existence in the GTP-bound form .

Structure and Function

DIRAS1 shares structural similarities with other members of the Ras superfamily, which are known for their role in intracellular signaling networks. However, DIRAS1 has unique amino acid substitutions in regions critical for GTP hydrolysis and interaction with RAF kinase, distinguishing it from other Ras proteins . These substitutions result in DIRAS1’s low GTPase activity and its predominant GTP-bound state .

The primary function of DIRAS1 is as a tumor suppressor. Unlike other Ras proteins that generally act as positive growth regulators, DIRAS1 inhibits cell growth and proliferation . This unique function is attributed to its structural differences and its role in regulating cell development in a manner distinct from other Ras family members .

Expression and Localization

DIRAS1 is highly expressed in heart and brain tissues . It is involved in various cellular processes, including signal transduction and the positive regulation of MAP kinase activity . The protein is localized to the plasma membrane, where it interacts with other signaling molecules to exert its effects .

Clinical Significance

DIRAS1 has been linked to several types of cancer, including glioblastoma, colorectal cancer, renal cell carcinoma, and ovarian cancer . Its role as a tumor suppressor makes it a potential target for cancer therapy. Understanding the mechanisms by which DIRAS1 inhibits cell growth could lead to the development of novel therapeutic strategies for cancer treatment.

Recombinant DIRAS1

Human recombinant DIRAS1 is produced using recombinant DNA technology, which involves inserting the DIRAS1 gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant protein can be used in various research applications, including studying its structure and function, investigating its role in cancer, and developing potential therapeutic agents.

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