DENR Human

Density-Regulated Protein Human Recombinant
Cat. No.
BT4252
Source
E.coli.
Synonyms
Density-regulated protein, DRP, Protein DRP1, Smooth muscle cell-associated protein 3,
SMAP-3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

DENR Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 218 amino acids (1-198) and having a molecular mass of 24.3 kDa.
The DENR is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
DENR participates in the translation of target mRNAs by identifying and attaching to the initiation codon. This protein influences the translation pattern of specific cancer-related mRNAs when it interacts with the translational initiation complex through the oncogene MCTS1.
Description
Recombinant human DENR protein, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 218 amino acids (residues 1-198) and has a molecular weight of 24.3 kDa. A 20 amino acid His-Tag is fused to the N-terminus of the protein. Purification is achieved using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The DENR solution (0.5 mg/ml) is prepared in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 2 mM DTT, and 20% glycerol.
Stability
For optimal storage, refrigerate at 4°C if the entire vial will be used within 2-4 weeks. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 85% as assessed by SDS-PAGE.
Synonyms
Density-regulated protein, DRP, Protein DRP1, Smooth muscle cell-associated protein 3,
SMAP-3.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAADISESSG ADCKGDPRNS AKLDADYPLR VLYCGVCSLP TEYCEYMPDV AKCRQWLEKN FPNEFAKLTV ENSPKQEAGI SEGQGTAGEE EEKKKQKRGG RGQIKQKKKT VPQKVTIAKI PRAKKKYVTR VCGLATFEID LKEAQRFFAQ KFSCGASVTG EDEIIIQGDF TDDIIDVIQE KWPEVDDDSI EDLGEVKK

Product Science Overview

Structure and Expression

The Density-Regulated Protein consists of 198 amino acids and is highly expressed in the heart and skeletal muscle, with moderate expression in the brain, placenta, liver, and pancreas. It is weakly expressed in the lung and kidney . The protein is up-regulated with increasing cell density by HNRNPD and is also up-regulated in ovarian and breast cancer cells by ERBB2 overexpression .

Function

The primary function of the Density-Regulated Protein is to form a complex with MCTS1 (Malignant T Cell Amplified Sequence 1) to promote translation reinitiation. This process involves the small ribosomal subunit remaining attached to the mRNA following the termination of translation of a regulatory upstream ORF (uORF) and resuming scanning on the same mRNA molecule to initiate translation of a downstream ORF, usually the main ORF (mORF) .

The MCTS1/DENR complex is pivotal to two linked mechanisms essential for translation reinitiation:

  1. Dissociation of deacylated tRNAs from post-termination 40S ribosomal complexes during ribosome recycling.
  2. Recruitment of aminoacylated initiator tRNA to the P site of 40S ribosomes for a new round of translation in an EIF2-independent manner .

This regulatory mechanism governs the translation of more than 150 genes whose translation reinitiation is MCTS1/DENR complex-dependent .

Recombinant Production

Recombinant Human Density-Regulated Protein is produced using Escherichia coli expression systems. The recombinant protein is typically purified to a high degree of purity (>85%) and is suitable for various applications such as SDS-PAGE and mass spectrometry (MS) . The recombinant form retains the functional properties of the native protein, making it valuable for research and therapeutic applications.

Clinical Relevance

The expression of Density-Regulated Protein is up-regulated in certain cancer cells, such as ovarian and breast cancer, indicating its potential role in cancer biology . Understanding the function and regulation of this protein could provide insights into novel therapeutic targets for cancer treatment.

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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