Dengue Premembrane
Description
Recombinant dengue 2 premembrane is a whole length peptide expressed from E. coil with 18kDa in size. PreM is considered as a signal peptide responsible for dengue envelope translocation in cells. Experiment showed it can elicit protective immune response, it is considered as a potentialtarget for vaccine development. Recombinant dengue 2 PreM is fused with a 6xHis tag.
Product Specs
Product Science Overview
Dengue virus (DENV) is a mosquito-borne virus that causes dengue fever, a significant public health concern in tropical and subtropical regions. There are four distinct serotypes of the dengue virus, namely DENV-1, DENV-2, DENV-3, and DENV-4. Among these, DENV-2 is known for its potential to cause severe disease manifestations, including dengue hemorrhagic fever and dengue shock syndrome.
The dengue virus is an enveloped virus with a single-stranded positive-sense RNA genome. The genome encodes three structural proteins: the capsid ©, premembrane (prM), and envelope (E) proteins, as well as seven non-structural proteins. The premembrane (prM) protein plays a crucial role in the virus’s life cycle, particularly in the assembly and maturation of viral particles.
The prM protein is initially produced as a precursor protein that undergoes cleavage to form the mature membrane (M) protein. This cleavage is essential for the virus to become infectious. The prM protein, in its uncleaved form, associates with the envelope (E) protein to form immature viral particles. These immature particles are non-infectious and require cleavage of the prM protein by host cell proteases to become mature and infectious.
Recombinant technology has been employed to study the function of the prM protein and its role in the viral life cycle. By co-expressing the prM and E proteins, researchers can generate recombinant virus-like particles (VLPs) that are biophysically and antigenically similar to infectious virions . These VLPs are valuable tools for studying the assembly, maturation, and immunogenic properties of the virus.
The recombinant prM protein has several important applications:
- Vaccine Development: Recombinant VLPs containing the prM and E proteins are being explored as potential vaccine candidates. These VLPs can elicit an immune response without the risk of causing disease, making them a safer alternative to live-attenuated vaccines.
- Serodiagnostic Antigens: Recombinant prM and E proteins can be used as antigens in serological assays to detect antibodies against the dengue virus. This is crucial for diagnosing dengue infection and monitoring immune responses in vaccinated individuals.
- Functional Studies: By studying recombinant prM proteins, researchers can gain insights into the mechanisms of viral assembly, maturation, and entry into host cells. This knowledge can inform the development of antiviral therapies targeting these processes.
