Dengue Polyvalent ELISA
Protein is >90% pure as determined by 12% PAGE (coomassie staining).
Description
Polyvalent dengue antigens are 18kDa proteins containing subtype 1,2,3 and 4 and each of them has equal amount protein. They are a new group of antigens specifically designed for ELISA assay. All the recombinant peptides are fused with a 6xHis Tag. Polyvalent dengue is purified by proprietary chromatographic technique.
Product Specs
Product Science Overview
Dengue fever, caused by the dengue virus (DENV), is a mosquito-borne viral infection prevalent in tropical and subtropical regions. The virus has four distinct serotypes (DENV-1, DENV-2, DENV-3, and DENV-4), and infection with one serotype provides lifelong immunity to that serotype but not to the others. Consequently, individuals can be infected multiple times, leading to severe complications such as dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS).
Accurate and timely diagnosis of dengue infection is crucial for patient management and outbreak control. Traditional diagnostic methods include virus isolation, reverse transcription-polymerase chain reaction (RT-PCR), and serological tests. Among these, enzyme-linked immunosorbent assay (ELISA) is widely used due to its simplicity, cost-effectiveness, and ability to handle large sample volumes.
Polyvalent Dengue Antigen-I for ELISA is a recombinant protein used as a diagnostic tool to detect dengue virus infections. This antigen is designed to be recognized by antibodies produced in response to all four dengue virus serotypes, making it a versatile tool for serological assays.
The recombinant Polyvalent Dengue Antigen-I is produced using genetic engineering techniques. The genes encoding the antigenic regions of the dengue virus are cloned into an expression vector, which is then introduced into a suitable host cell, such as Escherichia coli or yeast. The host cells express the recombinant protein, which is subsequently purified and used in ELISA kits.
ELISA is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones. In the context of dengue diagnosis, the Polyvalent Dengue Antigen-I is coated onto the wells of a microplate. When a patient’s serum sample is added to the wells, any dengue-specific antibodies present in the serum will bind to the antigen. After washing away unbound substances, a secondary antibody conjugated to an enzyme is added, which binds to the dengue-specific antibodies. A substrate is then added, and the enzyme catalyzes a colorimetric reaction, producing a measurable signal proportional to the amount of dengue-specific antibodies in the sample.
- Broad Detection Range: Capable of detecting antibodies against all four dengue virus serotypes.
- High Sensitivity and Specificity: Provides accurate results, reducing the likelihood of false positives and negatives.
- Cost-Effective: Suitable for large-scale screening in endemic regions.
- Ease of Use: Simple procedure that can be performed in standard laboratory settings.
