Dengue-HRP
Description
Recombinant Dengue-HRP is genetically designed dengue protein conjugated to Horseradish peroxidase which detects low titer dengue antibodies having a sharp OD value compared to the control with optimal dilutions. The proprietary Dengue-HRP protein sequence is designed for broad reactions to specific antibodies produced from the different serotypes of dengue infection. Potential applications for Dengue-HRP include indirect ELISA for the detection of dengue IgG or MAC ELISA for the detection of dengue IgM.
Product Specs
Product Science Overview
Dengue virus (DENV) is a mosquito-borne virus belonging to the Flaviviridae family and the Flavivirus genus . It is responsible for causing dengue fever, a disease prevalent in tropical and subtropical regions . The virus has four distinct serotypes (DENV-1, DENV-2, DENV-3, and DENV-4), each capable of causing the full spectrum of disease . Dengue fever symptoms range from mild flu-like symptoms to severe conditions such as dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) .
Horseradish peroxidase (HRP) is an enzyme found in the roots of the horseradish plant (Armoracia rusticana) . It is a metalloenzyme that catalyzes the oxidation of various organic substrates by hydrogen peroxide . HRP is widely used in biochemistry applications due to its ability to produce a detectable signal when conjugated to other molecules . It is commonly used in techniques such as ELISA (enzyme-linked immunosorbent assay), western blotting, and immunohistochemistry .
Recombinant proteins are proteins produced by host cells that have been genetically engineered to carry a gene of interest . The process involves inserting recombinant DNA into a host cell, which then transcribes and translates the DNA to produce the desired protein . This technology allows for the production of proteins with specific characteristics and is widely used in pharmaceuticals, biotechnology, and research .
The term “Dengue Horseradish Peroxidase Recombinant” likely refers to a recombinant protein that combines elements of the dengue virus and horseradish peroxidase. This recombinant protein could be used in various diagnostic and research applications. For example, it could be used in ELISA tests to detect dengue virus antibodies in patient samples. By conjugating HRP to dengue virus antigens, researchers can create a sensitive and specific assay for diagnosing dengue fever.
The production of such recombinant proteins involves several steps:
- Gene Cloning: The gene encoding the desired protein (e.g., a dengue virus antigen) is cloned into a suitable vector.
- Transformation: The vector is introduced into a host cell (e.g., E. coli or yeast) that will produce the recombinant protein.
- Expression: The host cells are cultured under conditions that promote the expression of the recombinant protein.
- Purification: The recombinant protein is purified from the host cells using various techniques such as affinity chromatography.
- Conjugation: The purified protein is conjugated to HRP to create the final product.
Recombinant HRP has been successfully produced in various host systems, including E. coli and yeast . These systems offer advantages such as high yield and ease of genetic manipulation . However, producing functional HRP can be challenging due to the need for proper folding and assembly of the heme prosthetic group .
