Dengue Envelope-2 & 4
Description
Dengue Type-2 and 4 is purified by proprietary chromatographic technique.
Product Specs
Product Science Overview
Dengue virus (DENV) is a member of the Flaviviridae family, which includes several other medically significant viruses. Dengue virus is transmitted through the bites of infected mosquitoes, primarily Aedes aegypti and Aedes albopictus. There are four distinct serotypes of the dengue virus: DENV-1, DENV-2, DENV-3, and DENV-4. Each serotype is sufficiently different that infection with one does not provide immunity against the others, leading to the possibility of sequential infections and severe disease manifestations such as dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS).
The envelope (E) protein of the dengue virus is a structural protein that plays a crucial role in the virus’s ability to infect host cells. It mediates the attachment of the virus to the host cell membrane and facilitates the fusion of the viral and cellular membranes, allowing the viral genome to enter the host cell. The E protein is also a major target for neutralizing antibodies, making it a key component in vaccine development.
The recombinant fusion protein of Dengue Virus Subtype 2 & 4 fused Envelope 52kDa is a genetically engineered protein that combines elements from the envelope proteins of both DENV-2 and DENV-4. This fusion protein is designed to elicit an immune response that targets both serotypes, potentially providing broader protection against dengue virus infections.
The production of recombinant dengue virus envelope proteins typically involves the use of bacterial or yeast expression systems. For example, the E. coli expression system is commonly used due to its ability to produce large quantities of recombinant protein. The recombinant protein is then purified using techniques such as affinity chromatography, which exploits the protein’s affinity for a specific ligand attached to a chromatography matrix.
Recombinant dengue virus envelope proteins have several important applications:
- Vaccine Development: The fusion protein can be used as an antigen in vaccine formulations to induce an immune response against multiple dengue virus serotypes.
- Diagnostic Tools: The protein can be used in serological assays to detect antibodies against dengue virus in patient samples.
- Research: The recombinant protein is valuable for studying the structure and function of the dengue virus envelope protein, as well as for screening potential antiviral compounds.
