Dengue Envelope-1, Insect

Dengue Virus Subtype 1 Recombinant, Insect Cells
Cat. No.
BT2492
Source
Insect cells.
Synonyms
Appearance
Sterile filtered colorless solution.
Purity
Protein is >95% pure as determined by 12.5% SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Recombinant Dengue Virus Subtype 1 produced in Insect Cells is a polypeptide chain containing amino acids 2-395 and having a molecular weight of 44.8kDa. Dengue Envelope-1 is purified by proprietary chromatographic technique.

Product Specs

Introduction
Dengue fever is caused by four closely related virus serotypes belonging to the genus Flavivirus, family Flaviviridae. Each serotype is distinct, lacking cross-protection, which allows for epidemics caused by multiple serotypes (hyperendemicity). Research involving cell culture and mice has demonstrated the specific antiviral activity of Morpholino antisense oligos against Dengue virus.
Description
Recombinant Dengue Virus Subtype 1, produced in insect cells, is a polypeptide chain encompassing amino acids 2-395. This protein has a molecular weight of 44.8kDa. Purification of the Dengue Envelope-1 protein is achieved using a proprietary chromatographic technique.
Physical Appearance
A sterile, colorless solution without any visible particles.
Formulation
Dengue Envelope-1 protein is supplied in a solution of 1xD-PBS with a pH of 7.3. The solution also contains 0.099% Thimerosal, 1µg/ml each of Leupeptin, Aprotinin, and Pepstatin A, along with 2.5% D-trehalose.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE analysis using a 12.5% gel.
Source
Insect cells.

Product Science Overview

Introduction to Dengue Virus

Dengue virus (DENV) is a mosquito-borne virus that belongs to the Flaviviridae family and the genus Flavivirus. There are four distinct serotypes of the dengue virus: DENV-1, DENV-2, DENV-3, and DENV-4. Each serotype can cause a spectrum of diseases ranging from mild dengue fever to severe dengue hemorrhagic fever and dengue shock syndrome .

Dengue Virus Subtype 1 (DENV-1)

DENV-1 is one of the four serotypes of the dengue virus. It is known for causing predominant infections and has been responsible for several outbreaks worldwide. The virus is transmitted to humans through the bite of infected Aedes mosquitoes, primarily Aedes aegypti and Aedes albopictus .

Recombinant Dengue Virus

Recombinant dengue virus refers to the virus that has been genetically engineered to express certain proteins or antigens. This is often done to study the virus’s behavior, develop vaccines, or produce diagnostic tools. Recombinant technology allows for the production of viral proteins in various host systems, including bacteria, yeast, mammalian cells, and insect cells .

Insect Cells as Expression Systems

Insect cells are commonly used as expression systems for producing recombinant proteins. The baculovirus expression vector system (BEVS) is a popular method for producing high levels of recombinant proteins in insect cells. This system involves the use of baculovirus, a virus that infects insects, to introduce the gene of interest into insect cells. The cells then produce the desired protein .

Production of DENV-1 Recombinant Proteins in Insect Cells

The production of DENV-1 recombinant proteins in insect cells involves several steps:

  1. Gene Cloning: The gene encoding the desired DENV-1 protein is cloned into a baculovirus transfer vector.
  2. Transfection: The recombinant baculovirus DNA is transfected into insect cells, typically Spodoptera frugiperda (Sf9) cells.
  3. Protein Expression: The insect cells are infected with the recombinant baculovirus, leading to the expression of the DENV-1 protein.
  4. Protein Purification: The expressed protein is purified from the insect cell culture using various chromatographic techniques .
Applications of DENV-1 Recombinant Proteins

Recombinant DENV-1 proteins produced in insect cells have several applications:

  • Vaccine Development: These proteins can be used to develop vaccines against DENV-1 by eliciting an immune response in the host.
  • Diagnostic Tools: Recombinant proteins can be used to develop diagnostic assays for detecting DENV-1 infections.
  • Research: These proteins are valuable tools for studying the structure, function, and immunogenicity of the dengue virus .

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