DEFB118 Human

Beta Defensin 118 (BD118), also known as DEFB118, is a member of the beta-defensin family, which consists of small cationic peptides known for their antimicrobial properties and immune-modulatory functions. These peptides play a crucial role in the innate immune response, providing protection against a wide range of microbial pathogens.

The DEFB118 gene is located on the long arm of chromosome 20, clustered with other beta-defensin genes . The gene’s expression is regulated by androgen, and the encoded protein binds to sperm, exhibiting antibacterial activity . The human recombinant BD118 is produced through genetic engineering techniques, typically in Escherichia coli (E. coli), and is a single, non-glycosylated polypeptide chain containing 126 amino acids with a molecular mass of approximately 13.8 kDa .

Human recombinant BD118 is generated using recombinant DNA technology. The gene encoding BD118 is inserted into an expression vector, which is then introduced into E. coli cells. These cells are cultured, and the BD118 protein is expressed and subsequently purified using chromatographic techniques. The recombinant protein often includes a His-tag at the N-terminus to facilitate purification .

Beta Defensin 118 plays a significant role in the innate immune system. It exhibits antibacterial activity against a variety of microorganisms, including Escherichia coli. The protein’s ability to bind to sperm suggests a role in reproductive biology, potentially protecting sperm from microbial infections .

The antimicrobial and immune-modulatory properties of BD118 make it a promising candidate for therapeutic applications. Research is ongoing to explore its potential in treating various inflammatory and infectious diseases. Human recombinant BD118 offers a valuable tool for studying these properties and developing new therapeutic interventions .