DEFB116 Human

Beta-defensins are small, cationic peptides characterized by their beta-sheet structure stabilized by disulfide bonds. DEFB116, like other beta-defensins, exhibits antimicrobial activity, which is vital for the host defense mechanism . The gene encoding DEFB116 is located on chromosome 20 and is densely clustered with other beta-defensin genes .

The expression of DEFB116 is regulated by various factors, including microbial components and inflammatory cytokines. This regulation ensures that DEFB116 is produced in response to infection or inflammation, thereby contributing to the body’s defense against pathogens .

Recombinant human DEFB116 is produced using Escherichia coli (E. coli) expression systems. The recombinant protein is often fused with a His-tag at the N-terminus to facilitate purification . The recombinant DEFB116 protein is typically used in research to study its antimicrobial properties and potential therapeutic applications .

Recombinant DEFB116 is valuable in various research applications, including:

• Antimicrobial assays: To evaluate its effectiveness against different microbial strains.
• Immunological studies: To understand its role in the immune response.
• Drug development: As a potential candidate for developing new antimicrobial agents .

Recombinant DEFB116 should be stored at 4°C for short-term use and at -20°C for long-term storage. It is essential to avoid repeated freeze-thaw cycles to maintain its stability and activity .