DDR2 Human

Discoidin Domain Receptor Tyrosine Kinase 2 Human Recombinant
Cat. No.
BT15359
Source
Sf9, Baculovirus cells.
Synonyms
Discoidin domain-containing receptor 2, DDR2, MIG20a, NTRKR3, TKT, TYR010, Discoidin Domain Receptor Tyrosine Kinase 2, CD167 antigen-like family member B, Discoidin domain-containing receptor tyrosine kinase 2, Neurotrophic tyrosine kinase, receptor-related 3, Receptor protein-tyrosine kinase TKT, Tyrosine-protein kinase TYRO10, CD_antigen: CD167b.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

DDR2 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 386 amino acids (22-399a.a) and having a molecular mass of 43.7kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
DDR2 is fused to 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Discoidin Domain Receptor Tyrosine Kinase 2 (DDR2) is a member of the discoidin-like domain containing subfamily of receptor tyrosine kinases, primarily expressed in mesenchymal cells. Unlike most RTKs, DDR2's ligand is fibrillar collagen, not a growth factor-like peptide. It plays a crucial role in various cellular processes, including cell differentiation, extracellular matrix remodeling, cell migration, and cell proliferation. DDR2 is also vital for normal bone development.
Description
Recombinant human DDR2, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain comprising 386 amino acids (22-399a.a). It has a molecular mass of 43.7kDa, although it appears as a band at approximately 40-57kDa on SDS-PAGE. The protein features an 8 amino acid His-tag fused at its C-terminus and undergoes purification through proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
The DDR2 solution is provided at a concentration of 0.5mg/ml and contains Phosphate Buffered Saline (pH 7.4) along with 10% glycerol.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For longer periods, it is recommended to store the solution frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Multiple freeze-thaw cycles should be avoided.
Purity
The purity of the protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Discoidin domain-containing receptor 2, DDR2, MIG20a, NTRKR3, TKT, TYR010, Discoidin Domain Receptor Tyrosine Kinase 2, CD167 antigen-like family member B, Discoidin domain-containing receptor tyrosine kinase 2, Neurotrophic tyrosine kinase, receptor-related 3, Receptor protein-tyrosine kinase TKT, Tyrosine-protein kinase TYRO10, CD_antigen: CD167b.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
KAQVNPAICR YPLGMSGGQI PDEDITASSQ WSESTAAKYG RLDSEEGDGA WCPEIPVEPD DLKEFLQIDL HTLHFITLVG TQGRHAGGHG IEFAPMYKIN YSRDGTRWIS WRNRHGKQVL DGNSNPYDIF LKDLEPPIVA RFVRFIPVTD HSMNVCMRVE LYGCVWLDGL VSYNAPAGQQ FVLPGGSIIY LNDSVYDGAV GYSMTEGLGQ LTDGVSGLDD FTQTHEYHVW PGYDYVGWRN ESATNGYIEI MFEFDRIRNF TTMKVHCNNM FAKGVKIFKE VQCYFRSEAS EWEPNAISFP LVLDDVNPSA RFVTVPLHHR MASAIKCQYH FADTWMMFSE ITFQSDAAMY NNSEALPTSP MAPTTYDPML KVDDSNTRLE HHHHHH.

Product Science Overview

Gene and Protein Structure

The DDR2 gene is located on chromosome 1 at the 1q23.3 band . The protein structure of DDR2 includes an N-terminal discoidin (DS) domain, a DS-like domain, a proline/glycine-rich juxtamembrane region, a transmembrane ™ region, an intracellular juxtamembrane region, and a C-terminal kinase domain . DDR2 undergoes phosphorylation following fibrillar collagen binding, which is sustained for over 24 hours .

Biological Functions

DDR2 plays a crucial role in various biological processes, including:

  • Collagen-Activated Tyrosine Kinase Receptor Signaling Pathway: DDR2 is activated by collagen binding, leading to signal transduction pathways involved in cell adhesion and proliferation .
  • Extracellular Matrix Organization: DDR2 is involved in the regulation of extracellular matrix disassembly and organization .
  • Wound Healing: DDR2 is known for its function in wound healing by engaging in cell adhesion and migration .
  • Tumor Growth and Invasiveness: DDR2 may regulate tumor growth and invasiveness, making it a potential target for cancer therapy .
Recombinant DDR2

Recombinant DDR2 refers to the artificially synthesized form of the DDR2 protein, produced using recombinant DNA technology. This technology allows for the production of large quantities of DDR2 for research and therapeutic purposes. Recombinant DDR2 is used in various studies to understand its role in cellular processes and its potential as a therapeutic target.

Clinical Significance

DDR2 has been implicated in several diseases, particularly cancer. Studies have shown that DDR2 can augment extracellular matrix remodeling, invasion, and drug resistance, facilitating tumor progression and metastasis . However, DDR2 also promotes apoptosis and suppresses tumor progression in various human cancers, making its role in cancer complex and multifaceted .

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