DCTD Human

dCMP Deaminase Human Recombinant
Cat. No.
BT4936
Source
Escherichia Coli.
Synonyms
Deoxycytidylate deaminase, EC 3.5.4.12, dCMP Deaminase, DCTD, MGC111062.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

DCTD Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 198 amino acids (1-178 a.a.) and having a molecular mass of 22.1 kDa. The DCTD is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Deoxycytidylate deaminase (DCTD) is an enzyme that plays a crucial role in nucleotide metabolism. It catalyzes the deamination of deoxycytidine monophosphate (dCMP) to deoxyuridine monophosphate (dUMP). This reaction is essential for providing dUMP, a key substrate required for the synthesis of thymidine nucleotides, which are essential components of DNA. DCTD is part of the cytidine and deoxycytidylate deaminase protein family and functions as a homohexamer. Its activity is dependent on zinc, which acts as a cofactor.
Description
This product consists of the recombinant human DCTD protein, expressed in E. coli and purified to a high degree. The protein is a single, non-glycosylated polypeptide chain with a molecular weight of 22.1 kDa. It encompasses amino acids 1 to 178 of the native DCTD sequence and includes an N-terminal His-tag (20 amino acids) to facilitate purification. The protein has been purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterilized by filtration.
Formulation
This DCTD protein solution is provided at a concentration of 0.5 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8), 1 mM DTT, 1 mM EDTA, and 20% glycerol.
Stability
For optimal storage, the product should be kept refrigerated at 4°C if it will be used within 2-4 weeks. For long-term storage, it is recommended to freeze the solution at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein such as HSA or BSA (0.1%) is advised. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of this protein is greater than 90%, as assessed by SDS-PAGE analysis.
Synonyms
Deoxycytidylate deaminase, EC 3.5.4.12, dCMP Deaminase, DCTD, MGC111062.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSEVSCKKRD DYLEWPEYFM AVAFLSAQRS KDPNSQVGAC IVNSENKIVG IGYNGMPNGC SDDVLPWRRT AENKLDTKYP YVCHAELNAI MNKNSTDVKG CSMYVALFPC NECAKLIIQA GIKEVIFMSD KYHDSDEATA ARLLFNMAGV TFRKFIPKCS KIVIDFDSIN SRPSQKLQ.

Product Science Overview

Structure and Function

dCMP deaminase is an allosteric enzyme that typically exists as a homohexamer. It belongs to the cytidine and deoxycytidylate deaminase protein family . The enzyme uses zinc as a cofactor to facilitate the deamination process . The reaction it catalyzes is essential for maintaining the balance of nucleotide pools within the cell, particularly the relative concentrations of dCDP and dTTP .

Genetic and Molecular Information

The gene encoding dCMP deaminase in humans is known as DCTD and is located on chromosome 4q35.1 . The enzyme consists of 178 amino acids and has a molecular weight of approximately 22.1 kDa . The recombinant form of this enzyme is often produced in E. coli and purified using conventional chromatography techniques .

Biological Significance

The primary function of dCMP deaminase is to provide the nucleotide substrate (dUMP) for thymidylate synthase, which is crucial for DNA synthesis and repair . By converting dCMP to dUMP, the enzyme helps regulate the availability of thymidine nucleotides, which are necessary for DNA replication and cell division .

Applications in Research

Recombinant human dCMP deaminase is widely used in biochemical and molecular biology research. It is often utilized to study nucleotide metabolism, enzyme kinetics, and the regulation of nucleotide pools within cells . The recombinant enzyme is also valuable for investigating the mechanisms of enzyme action and the effects of various inhibitors on its activity .

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THE BIOTEK
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