DCPS Human

The Decapping Enzyme, Scavenger (DCPS) is a crucial component in the mRNA decay pathway. This enzyme plays a significant role in the final step of the 3’ end mRNA decay process, ensuring the proper degradation of mRNA molecules. The human recombinant form of this enzyme is produced using advanced biotechnological methods, typically involving expression in E. coli.

The human recombinant DCPS is a single, non-glycosylated polypeptide chain consisting of 357 amino acids. It has a molecular mass of approximately 40.8 kDa. The enzyme is often fused to a 20 amino acid His-tag at the N-terminus to facilitate purification through chromatographic techniques .

DCPS is essential for the complete degradation of mRNAs. It hydrolyzes the residual m7GpppN cap structure after the mRNA has been degraded by the exosome. This process releases m7GMP, ensuring that the cap structure is not left intact, which could otherwise interfere with cellular processes .

The recombinant form of DCPS is expressed in E. coli and purified using proprietary chromatographic techniques. The enzyme is typically formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 20% glycerol, and 1 mM DTT. This formulation helps maintain the stability and activity of the enzyme .

The human recombinant DCPS is used extensively in research to study mRNA decay mechanisms. It is also employed in various biochemical assays to understand the role of mRNA decapping in gene expression regulation. The enzyme’s ability to hydrolyze cap structures makes it a valuable tool in molecular biology and genetic research .

For short-term storage, the enzyme can be kept at 4°C. For long-term storage, it is recommended to store the enzyme at -20°C. To prevent degradation, it is advisable to avoid multiple freeze-thaw cycles. Adding a carrier protein, such as 0.1% HSA or BSA, can further enhance the enzyme’s stability during storage .