DCAF7 Human
DDB1- and CUL4-associated factor 7, WD repeat-containing protein 68, WD repeat-containing protein An11 homolog, DCAF7, HAN11, WDR68, AN11, SWAN-1.
Description
DCAF7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 298 amino acids (19-295 a.a) and having a molecular mass of 33.6kDa.
DCAF7 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
DDB1- and CUL4-associated factor 7, WD repeat-containing protein 68, WD repeat-containing protein An11 homolog, DCAF7, HAN11, WDR68, AN11, SWAN-1.
MGSSHHHHHH SSGLVPRGSH MVYAMNWSVR PDKRFRLALG SFVEEYNNKV QLVGLDEESS EFICRNTFDH PYPTTKLMWI PDTKGVYPDL LATSGDYLRV WRVGETETRL ECLLNNNKNS DFCAPLTSFD WNEVDPYLLG TSSIDTTCTI WGLETGQVLG RVNLVSGHVK TQLIAHDKEV YDIAFSRAGG GRDMFASVGA DGSVRMFDLR HLEHSTIIYE DPQHHPLLRL CWNKQDPNYL ATMAMDGMEV VILDVRVPCT PVARLNNHRA CVNGIAWAPH SSCHICTAAD DHQALIWD.
Product Science Overview
DCAF7 is a scaffold protein that is essential for the formation of protein complexes involved in kinase signaling. It has been shown to interact with DYRK1A, a kinase located on chromosome 21, which is significant in the context of Down syndrome . The protein is also involved in craniofacial development, acting upstream of the EDN1 pathway and is required for the formation of the upper jaw equivalent .
Recent studies have highlighted the importance of DCAF7 in hematopoiesis. Mutations in DCAF7 have been detected in a subset of patients with Myeloid Leukemia in Down syndrome. The absence of DCAF7 in hematopoietic cell lines leads to cell cycle arrest and decreased proliferation . This protein is crucial for the differentiation and proliferation of hematopoietic stem cells (HSCs) and progenitor cells. In conditional knockout mouse models, the deletion of DCAF7 resulted in a decrease in white blood cells, hemoglobin, and platelet counts, indicating its essential role in hematopoiesis .
DCAF7 is associated with several diseases, including Dystonia 35, Childhood-Onset, and Noonan Syndrome 3 . Its role in kinase signaling and protein folding pathways makes it a potential target for therapeutic interventions. The protein’s involvement in craniofacial development and hematopoiesis further underscores its clinical significance.
Recombinant human DCAF7 is produced using advanced biotechnological methods. The gene encoding DCAF7 is cloned into an expression vector, which is then introduced into a suitable host cell line, such as E. coli or CHO cells. The recombinant protein is expressed, purified, and characterized to ensure its functionality and structural integrity. This recombinant protein is used in various research applications, including studies on protein-protein interactions, kinase signaling pathways, and hematopoiesis.
