DBI Human

DBI Human Recombinant
Cat. No.
BT3883
Source
Escherichia Coli.
Synonyms

ACBP, ACBD1, CCK-RP, EP, DBI, EP, MGC70414, Acyl-CoA-binding protein, GABA receptor modulator.

Appearance
Sterile filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

DBI Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 107 amino acids (1-87a.a.) and having a molecular mass of 12.2kDa. DBI protein is fused to a 20 amino acid His tag at N-terminus and is purified by standard chromatography.

Product Specs

Introduction
DBI, also known as Acyl-CoA-binding protein isoform 2, is a binding inhibitor regulated by hormones. It plays a role in lipid metabolism and displaces beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors in brain synapses. DBI expression increases significantly during adipocyte differentiation. It acts as an acyl-CoA pool former and regulates long-chain fatty acid (LCFA) metabolism in peripheral tissues.
Description
Recombinant Human DBI, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 107 amino acids (1-87a.a.) and has a molecular weight of 12.2 kDa. The DBI protein is fused to a 20 amino acid His tag at the N-terminus and purified using standard chromatography techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The Human DBI solution is supplied in 20mM Tris-HCl (pH 8), 0.1M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 95% as determined by SDS-PAGE analysis.
Synonyms

ACBP, ACBD1, CCK-RP, EP, DBI, EP, MGC70414, Acyl-CoA-binding protein, GABA receptor modulator.

Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSQAEFEKAA EEVRHLKTKP SDEEMLFIYG HYKQATVGDI NTERPGMLDF TGKAKWDAWN ELKGTSKEDA MKAYINKVEE LKKKYGI.

Product Science Overview

Structure and Function

DBI is a small protein that binds to long-chain acyl-CoA molecules, playing a crucial role in lipid metabolism. It is also known for its ability to bind to the benzodiazepine-binding site of the γ-aminobutyric acid (GABA) type A receptor (GABA_A_R), thereby regulating its activity . This dual functionality makes DBI a significant protein in both metabolic and neurological contexts.

Biological Roles

  1. Lipid Metabolism: DBI/ACBP binds to long-chain acyl-CoA molecules, which are essential intermediates in lipid metabolism. By regulating the availability of these molecules, DBI influences various metabolic pathways .

  2. Neurological Functions: DBI interacts with the GABA_A receptor, which is a major inhibitory neurotransmitter receptor in the brain. This interaction modulates the receptor’s activity, affecting neuronal excitability and various neurological processes .

  3. Steroid Synthesis: DBI binds to the peripheral benzodiazepine receptor (also known as the mitochondrial benzodiazepine receptor) and facilitates the transport of cholesterol to the inner mitochondrial membrane. This process is crucial for the synthesis of steroids .

Clinical Significance

  1. Cancer Research: DBI is highly expressed in certain types of cancer, such as glioblastoma. It promotes tumor growth by providing long-chain acyl-CoA molecules to mitochondria for fatty acid oxidation. Reduced expression of DBI has been associated with slower proliferation of glioblastoma cells .

  2. Neurogenesis: DBI is a regulator of neural progenitor and stem cell proliferation in adult neurogenic niches. This makes it a potential target for therapies aimed at enhancing neurogenesis and treating neurodegenerative diseases .

Applications of Recombinant DBI

The recombinant form of DBI is used in various research applications, including:

  1. Antibody Production: Recombinant DBI is used to produce monoclonal antibodies for research and diagnostic purposes. These antibodies can be used in techniques such as Western blotting and immunohistochemistry to study DBI expression and function .

  2. Functional Studies: Recombinant DBI allows researchers to study its biological functions in a controlled environment. This includes investigating its role in lipid metabolism, neurological processes, and cancer biology .

  3. Therapeutic Research: Understanding the functions of DBI and its interactions with other molecules can lead to the development of new therapeutic strategies for diseases related to lipid metabolism, neurological disorders, and cancer .

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Source
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THE BIOTEK
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