DARS Human

Aspartyl-tRNA Synthetase Human Recombinant

Cat. No.

BT25722

Source

E.coli.

Synonyms

Aspartyl-tRNA synthetase, Cell proliferation-inducing gene 40 protein, AspRS, aspartate tRNA ligase 1 cytoplasmic, EC 6.1.1.12.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 85% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

DARS Recombinant produced in E. coli is a single polypeptide chain containing 521 amino acids (1-501) and having a molecular mass of 59.3kDa.
DARS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

DARS, also known as aspartyl-tRNA synthetase, catalyzes a two-step reaction to attach an aspartate amino acid to its corresponding tRNA molecule. In the first step, the aspartate is activated by ATP, forming aspartyl-AMP. Subsequently, the aspartate moiety is transferred to the acceptor end of the tRNA.

Description

Recombinant DARS, produced in E. coli, is a single polypeptide chain with a molecular weight of 59.3 kDa. It consists of 521 amino acids, with the first 501 amino acids corresponding to the DARS protein. A 20 amino acid His-tag is fused to the N-terminus to facilitate purification using proprietary chromatographic techniques.

Physical Appearance

Clear, colorless solution that has been sterilized by filtration.

Formulation

The DARS solution is provided at a concentration of 1 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM DTT, and 40% glycerol.

Stability

For short-term storage (2-4 weeks), the DARS solution can be stored at 4°C. For long-term storage, it is recommended to store the solution at -20°C. Adding a carrier protein like 0.1% HSA or BSA is advisable for long-term storage. Avoid repeated freezing and thawing of the solution.

Purity

The purity of the DARS protein is greater than 85%, as determined by SDS-PAGE analysis.

Synonyms

Aspartyl-tRNA synthetase, Cell proliferation-inducing gene 40 protein, AspRS, aspartate tRNA ligase 1 cytoplasmic, EC 6.1.1.12.

Source

E.coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MPSASASRKS QEKPREIMDA AEDYAKERYG ISSMIQSQEK PDRVLVRVRD LTIQKADEVV WVRARVHTSR AKGKQCFLVL RQQQFNVQAL VAVGDHASKQ MVKFAANINK ESIVDVEGVV RKVNQKIGSC TQQDVELHVQ KIYVISLAEP RLPLQLDDAV RPEAEGEEEG RATVNQDTRL DNRVIDLRTS TSQAVFRLQS GICHLFRETL INKGFVEIQT PKIISAASEG GANVFTVSYF KNNAYLAQSP QLYKQMCICA DFEKVFSIGP VFRAEDSNTH RHLTEFVGLD IEMAFNYHYH EVMEEIADTM VQIFKGLQER FQTEIQTVNK QFPCEPFKFL EPTLRLEYCE ALAMLREAGV EMGDEDDLST PNEKLLGHLV KEKYDTDFYI LDKYPLAVRP FYTMPDPRNP KQSNSYDMFM RGEEILSGAQ RIHDPQLLTE RALHHGIDLE KIKAYIDSFR FGAPPHAGGG IGLERVTMLF LGLHNVRQTS MFPRDPKRLT P.

Product Science Overview

Introduction

Aspartyl-tRNA synthetase (AspRS) is an essential enzyme involved in the translation process of protein synthesis. It belongs to the family of aminoacyl-tRNA synthetases (AARSs), which are responsible for attaching amino acids to their corresponding tRNAs. This process is crucial for the accurate translation of mRNA into proteins. AspRS specifically catalyzes the attachment of aspartic acid to its cognate tRNA, forming aspartyl-tRNA, which is then used in the ribosome during protein synthesis.

Structure and Function

AspRS is a highly conserved enzyme found in all domains of life, including bacteria, archaea, and eukaryotes. In humans, AspRS is encoded by the DARS gene. The enzyme consists of several domains, including the catalytic domain responsible for the aminoacylation reaction and additional domains that may be involved in tRNA binding and enzyme regulation.

The primary function of AspRS is to ensure the correct pairing of aspartic acid with its corresponding tRNA. This process involves two main steps:

Activation of Aspartic Acid: AspRS catalyzes the reaction between aspartic acid and ATP, forming aspartyl-adenylate and releasing pyrophosphate.
Transfer to tRNA: The activated aspartic acid is then transferred to the 3’ end of the tRNA, forming aspartyl-tRNA.

Recombinant AspRS

Recombinant AspRS refers to the enzyme produced through recombinant DNA technology. This involves cloning the DARS gene into an expression vector, introducing it into a host organism (such as E. coli), and inducing the expression of the enzyme. The recombinant enzyme can then be purified for various applications, including biochemical studies, structural analysis, and therapeutic research.

Applications and Importance

AspRS plays a critical role in protein synthesis, and its malfunction can lead to various diseases. Mutations in the DARS gene have been associated with hypomyelination with brain stem and spinal cord involvement and leg spasticity (HBSL), a rare neurological disorder. Understanding the structure and function of AspRS is essential for developing potential therapeutic interventions for such conditions.

Recombinant AspRS is also valuable in research settings. It allows scientists to study the enzyme’s properties, investigate its interactions with other molecules, and explore its role in cellular processes. Additionally, recombinant AspRS can be used in high-throughput screening assays to identify potential inhibitors or modulators of the enzyme, which could lead to the development of new drugs.

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DARS Human

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