DACT3 Antibody

DACT3 is a 63 kDa protein that contains several important domains:

• Coiled-coil region: Located between amino acids 63-87, this region is involved in protein-protein interactions.
• Arginine-rich domain: Spanning amino acids 351-476, this domain is crucial for the protein’s function.
• PDZ-binding motif: Found at the C-terminus (amino acids 607-610), this motif is essential for binding to other proteins in the Wnt signaling pathway .

DACT3 is expressed in various tissues, including the cerebral cortex and uterus. Its expression is particularly significant in colorectal tumor cells, where it can initiate apoptosis .

DACT3 antagonizes the Wnt/β-catenin signaling pathway by interacting with Dishevelled (Dvl) proteins. This interaction prevents β-catenin from entering the nucleus, thereby inhibiting the transcription of Wnt target genes. In colorectal tumor cells, this inhibition leads to apoptosis, highlighting DACT3’s potential role as a tumor suppressor .

The Mouse Anti-Human DACT3 antibody is a polyclonal antibody that detects human and mouse DACT3 in direct ELISAs and Western blots. It is produced by immunizing mice with an E. coli-derived recombinant mouse DACT3 protein (Pro486-Lys587). The antibody is purified using antigen affinity purification, ensuring high specificity and minimal cross-reactivity with other proteins .

This antibody is widely used in various research applications, including:

• Western Blotting: To detect DACT3 in tissue lysates.
• ELISA: For quantifying DACT3 levels in different samples.
• Immunohistochemistry: To study the localization and expression patterns of DACT3 in tissues .

The antibody is typically supplied in a lyophilized form and should be reconstituted in sterile PBS. It is stable for 12 months when stored at -20 to -70°C and for 1 month at 2 to 8°C under sterile conditions after reconstitution. Repeated freeze-thaw cycles should be avoided to maintain its activity .