CYTL1 Human

Cytokine-Like 1 Human Recombinant
Cat. No.
BT30382
Source
Escherichia Coli.
Synonyms
Cytokine-like protein 1, Protein C17, CYTL1, C4orf4, C17.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CYTL1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (aa 23-136) containing 124 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 14.6kDa (calculated).

Product Specs

Introduction
Cytokine-like protein 1 (CYTL1) is a secreted protein primarily found in hematopoietic stem and progenitor cells (CD34+ cells) within the bone marrow and cord blood. Notably absent in CD34- cells, CYTL1 plays a crucial role in maintaining cartilage homeostasis, as evidenced by studies on Cytl1-/- knockout mice. These mice exhibit experimental osteoarthritic cartilage destruction, suggesting CYTL1's importance in cartilage health and its potential link to osteoarthritis.
Description
Recombinant human CYTL1, expressed in E. coli, is a non-glycosylated polypeptide chain encompassing amino acids 23-136. This includes a 10 amino acid N-terminal His tag, resulting in a protein with a molecular weight of 14.6 kDa. The protein is a single chain, ensuring purity and homogeneity.
Physical Appearance
White, lyophilized powder.
Formulation
The CYTL1 protein was lyophilized to a concentration of 0.5 mg/ml in a 20 mM Tris buffer with 50 mM NaCl, at a pH of 7.5. Prior to lyophilization, the solution underwent filtration through a 0.4 µm filter.
Solubility
To prepare a working stock solution, add deionized water to the lyophilized pellet to achieve a concentration of approximately 0.5 mg/ml. Allow the pellet to dissolve completely. It is essential to note that the provided CYTL1 is not sterile. Before using it in cell culture, filter the solution through an appropriate sterile filter.
Stability
Store the lyophilized CYTL1 protein at -20°C. Once reconstituted, aliquot the protein into smaller volumes to minimize freeze-thaw cycles. Reconstituted CYTL1 remains stable at 4°C for a limited period and shows no significant change after two weeks at this temperature.
Purity
The purity of CYTL1 is greater than 95.0%, as determined by SDS-PAGE analysis.
Synonyms
Cytokine-like protein 1, Protein C17, CYTL1, C4orf4, C17.
Source
Escherichia Coli.
Amino Acid Sequence
MKHHHHHHASTPPTCYSRMR ALSQEITRDF NLLQVSEPSE PCVRYLPRLY LDIHNYCVLD KLRDFVASPP CWKVAQVDSL KDKARKLYTI MNSFCRRDLV FLLDDCNALE YPIPVTTVLP DRQR.

Product Science Overview

Discovery and Expression

CYTL1 was first identified in CD34+ cells derived from bone marrow and cord blood . CD34+ cells are a sub-population of human mononuclear cells that function as hematopoietic stem/progenitor cells . The expression of CYTL1 is not found in mature hematopoietic cells that lack CD34 expression . This discovery was part of an immunogenomics-based screening platform aimed at identifying novel potential cytokines .

Biological Functions

CYTL1 has been shown to possess chemotactic activity, particularly towards monocytes and macrophages . It chemoattracts human monocytes via the CCR2B receptor and the ERK signaling pathway . Additionally, CYTL1 has been implicated in the regulation of chondrogenesis, cartilage homeostasis, and osteoarthritis progression . It modulates the expression of Sox9 and insulin-like growth factor 1, which are crucial for cartilage formation and maintenance .

Therapeutic Potential

Human recombinant CYTL1, produced through genetic engineering techniques, provides researchers with a valuable tool to study its biological activities and explore its therapeutic potential . CYTL1’s role in immune regulation and tissue repair makes it a promising candidate for developing new treatments for various conditions, including inflammatory arthritis, cardiac fibrosis, and certain types of cancer .

Research and Clinical Applications

The immunobiological functions of CYTL1 are still being explored, and its potential receptor(s) have not been fully identified . However, studies have shown that CYTL1 can significantly prevent inflammatory arthritis and is required for the maintenance of cartilage homeostasis . High CYTL1 expression has also been observed in cartilaginous tissues and in proteomics analyses of human osteoarthritic patients .

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