Cyclophilin F Rat Bioactive
Cyclophilin-F Rat Recombinant Bioactive
Cat. No.
BT3483
Source
Escherichia Coli.
Synonyms
Peptidyl-prolyl cis-trans isomerase F, mitochondrial, PPIase
F, Cyclophilin D, CyP-D, CypD, Cyclophilin F, Rotamase F.
Appearance
Sterile filtered colorless solution.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Cyclophilin F Rat Recombinant produced in E.Coli is a
single, non-glycosylated polypeptide chain containing 200 amino acids (30-206 a.a)
and having a molecular mass of 21.2Da.
Cyclophilin F is fused to a
23 amino acid His-tag at N-terminus & purified by proprietary
chromatographic techniques.
Product Specs
Introduction
PPIF, a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family, plays a crucial role in protein folding by catalyzing the cis-trans isomerization of proline imidic peptide bonds within oligopeptides. As a key component of the mitochondrial permeability transition pore in the inner mitochondrial membrane, PPIF activation is believed to be involved in triggering both apoptotic and necrotic cell death.
Description
Recombinant Cyclophilin F Rat, produced in E. coli, is a single, non-glycosylated polypeptide chain composed of 200 amino acids (specifically, residues 30-206). It has a molecular weight of 21.2 kDa. This Cyclophilin F variant is fused to a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
The product is a clear, sterile solution without any color.
Formulation
The Cyclophilin F protein solution is provided at a concentration of 1 mg/ml. It is formulated in a buffer consisting of Phosphate Buffered Saline (pH 7.4), 10% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For longer storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein like HSA or BSA (0.1%). It's important to avoid repeated freezing and thawing of the product.
Purity
The purity of the product is greater than 90%, as determined by SDS-PAGE analysis.
Biological Activity
The specific activity of the enzyme is measured as greater than 1,300 nmol/min/mg. This is determined by measuring the amount of enzyme required to cleave 1 nmol of the substrate suc-AAFP-PNA per minute at a temperature of 37°C in a Tris-HCl buffer at pH 8.0 using chymotrypsin.
Synonyms
Peptidyl-prolyl cis-trans isomerase F, mitochondrial, PPIase
F, Cyclophilin D, CyP-D, CypD, Cyclophilin F, Rotamase F.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSCSDGGAR GANSSSQNPL VYLDVGADGQ PLGRVVLELK ADVVPKTAEN
FRALCTGEKG FGYKGSTFHR VIPAFMCQAG DFTNHNGTGG KSIYGSRFPD ENFTLKHVGP GVLSMANAGP
NTNGSQFFIC TIKTDWLDGK HVVFGHVKEG MDVVKKIESF
GSKSGKTSKK IVITDCGQLS.
Product Science Overview
Molecular Properties
- Amino Acid Sequence: Cyclophilin-F (Rat Recombinant Bioactive) consists of 200 amino acids, specifically from positions 30 to 206 .
- Molecular Mass: The molecular mass of this recombinant protein is approximately 21.2 kDa .
- Tag: It is fused to a 23 amino acid His-tag at the N-terminus, which aids in purification .
Production and Purification
Biological Activity
Cyclophilin-F is a key component of the mitochondrial permeability transition pore (mPTP) in the inner mitochondrial membrane . The activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death . The specific activity of Cyclophilin-F is greater than 1,300 nmol/min/mg, defined as the amount of enzyme that cleaves 1 nmol of suc-AAPF-pNA per minute at 37°C in Tris-HCl pH 8.0 using chymotrypsin .
Applications and Storage
Cyclophilin-F (Rat Recombinant Bioactive) is used primarily for laboratory research purposes. It is not approved for use in humans or for clinical diagnosis . For storage, it is recommended to keep the protein at 4°C for short-term use (2-4 weeks) and at -20°C for long-term storage. It is advisable to avoid multiple freeze-thaw cycles to maintain protein stability .
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