Cyclophilin-E Antibody
Peptidyl-prolyl cis-trans isomerase E, PPIase E, Rotamase E, Cyclophilin-33, PPIE, peptidylprolyl isomerase E, CYP33, Cyclophilin E, CYP-33, MGC3736, MGC111222.
Description
Product Specs
Cyclophilin-E, a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family, catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides, accelerating protein folding. This protein possesses a highly conserved cyclophilin domain and an RNA-binding domain, contributing to its PPIase activity, protein folding capabilities, and RNA-binding activity. Notably, Cyclophilin-E features two RNA binding domains at its N-terminal region and a PPIase domain at its C-terminal region.
The formulation contains 1mg/ml of Cyclophilin-E antibody in a buffer consisting of PBS at pH 7.4, 10% Glycerol, and 0.02% Sodium Azide.
The Cyclophilin-E antibody has undergone rigorous testing through ELISA and Western blot analysis, ensuring its specificity and reactivity. However, due to variations in applications, it is recommended to titrate the reagent for each specific experiment to achieve optimal results.
Peptidyl-prolyl cis-trans isomerase E, PPIase E, Rotamase E, Cyclophilin-33, PPIE, peptidylprolyl isomerase E, CYP33, Cyclophilin E, CYP-33, MGC3736, MGC111222.
Cyclophilin-E antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT17E8AT.
Anti-human Cyclophilin-E mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Cyclophilin-E amino acids 1-301 purified from E. coli.
Mouse IgG1 heavy chain and κ light chain.
Product Science Overview
Cyclophilin-E (CypE), also known as Peptidyl-prolyl cis-trans isomerase E (PPIE), is a member of the cyclophilin family of proteins. Cyclophilins are peptidyl-prolyl cis-trans isomerases (PPIases) that catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides, facilitating protein folding and function .
Cyclophilin-E contains a highly conserved cyclophilin (CYP) domain and an RNA-binding domain . This dual functionality allows CypE to participate in both protein folding and RNA-binding activities. The protein is involved in various cellular processes, including protein folding, signal transduction, and immune response .
Antibodies targeting Cyclophilin-E, such as mouse anti-human Cyclophilin-E antibodies, are valuable tools in scientific research. These antibodies are used in several applications, including:
- Western Blot (WB): To detect and quantify Cyclophilin-E in protein samples.
- Immunohistochemistry (IHC): To visualize Cyclophilin-E expression in tissue sections.
- Immunocytochemistry (ICC): To study Cyclophilin-E localization in cultured cells.
- Enzyme-Linked Immunosorbent Assay (ELISA): To measure Cyclophilin-E levels in various samples.
- Immunoprecipitation (IP): To isolate Cyclophilin-E and its interacting partners from cell lysates .
Cyclophilins, including Cyclophilin-E, are implicated in various pathological states such as viral replication, inflammation, and cancer progression . Inhibition of Cyclophilin activity has been explored as a therapeutic strategy for treating diseases like hepatitis C virus (HCV) infection, human immunodeficiency virus (HIV) infection, and certain cancers . Non-immunosuppressive derivatives of cyclosporine A (CsA) have shown promise as Cyclophilin inhibitors, highlighting their potential as therapeutic agents .
