Introduction
Antibodies targeting CUB and Zona Pellucida-Like Domains 1 (CUZD1) have been shown to inhibit both the attachment and proliferation of ovarian cancer cells, suggesting a potential role for CUZD1 in these cellular processes. Furthermore, studies indicate that CUZD1 may play a role in uterine function during the later stages of pregnancy and/or in the activation of trypsin within pancreatic acinar cells.
Description
This recombinant human CUZD1 protein, expressed in HEK cells, consists of a single glycosylated polypeptide chain encompassing amino acids Glu25 to Ser568 (totaling 554 amino acids). It has a predicted molecular weight of 62.2 kDa and includes a 10 amino acid His tag fused to the C-terminus.
Physical Appearance
The product is provided as a sterile, white, lyophilized (freeze-dried) powder.
Formulation
The CUZD1 protein was subjected to filtration (0.4 µm) and subsequently lyophilized in a solution of phosphate buffered saline containing 5% (w/v) trehalose.
Solubility
To prepare a working stock solution, it is recommended to add deionized water to the lyophilized pellet, aiming for a final concentration of approximately 0.5 mg/ml. Allow sufficient time for the pellet to dissolve completely. Prior to use in cell culture, ensure sterility by filtering the reconstituted protein solution through an appropriate sterile filter.
Stability
The lyophilized protein should be stored at -20°C. To minimize freeze-thaw cycles, it is advisable to aliquot the reconstituted protein. While the reconstituted protein can be stored at 4°C for a limited period, it is generally stable for up to two weeks at this temperature without significant degradation.
Purity
Analysis by SDS-PAGE indicates a purity exceeding 95.0%.
Synonyms
CUB and Zona Pellucida-Like Domains 1, CUB and ZP Domain-Containing Protein 1, Transmembrane Protein UO-44, Estrogen Regulated Gene 1, ERG-1, UO-44, CUZD1.
Amino Acid Sequence
EAEGNASCTV SLGGANMAET HKAMILQLNP SENCTWTIER PENKSIRIIF SYVQLDPDGS CESENIKVFD GTSSNGPLLG QVCSKNDYVP VFESSSSTLT FQIVTDSARI QRTVFVFYYF FSPNISIPNC GGYLDTLEGS FTSPNYPKPH PELAYCVWHI QVEKDYKIKL NFKEIFLEID KQCKFDFLAI YDGPSTNSGL IGQVCGRVTP TFESSSNSLT VVLSTDYANS YRGFSASYTS IYAENINTTS LTCSSDRMRV IISKSYLEAF NSNGNNLQLK DPTCRPKLSN VVEFSVPLNG CGTIRKVEDQ SITYTNIITF SASSTSEVIT RQKQLQIIVK CEMGHNSTVE IIYITEDDVI QSQNALGKYN TSMALFESNS FEKTILESPY YVDLNQTLFV QVSLHTSDPN LVVFLDTCRA SPTSDFASPT YDLIKSGCSR DETCKVYPLF GHYGRFQFNA FKFLRSMSSV YLQCKVLICD SSDHQSRCNQ GCVSRSKRDI SSYKWKTDSI IGPIRLKRDR SASGNSGFQH ETHAEETPNQ PFNS HHHHHH HHHH.