CUL1 Human

Cullin-1 serves as a scaffold protein within the SCF complex. It organizes the SKP1-F-box protein and RBX1 subunits, facilitating the ubiquitination process. The SCF complex’s E3 ubiquitin ligase activity is dependent on the neddylation of the cullin subunit, although this may be dispensable for some in vitro reactions .

Recombinant human Cullin-1 is typically produced in various expression systems, including Escherichia coli (E. coli), Spodoptera frugiperda (Sf 21) cells, and wheat germ . The recombinant protein is often tagged with His-tag for purification purposes and is available in different formulations, such as carrier-free or with Bovine Serum Albumin (BSA) to enhance stability .

Recombinant Cullin-1 is used in various research applications, including:

• In vitro ubiquitination assays: To study the ubiquitination process and identify potential substrates.
• Protein-protein interaction studies: To investigate interactions within the SCF complex and with other cellular proteins.
• Structural biology: To determine the structure of the SCF complex and understand its mechanism of action.

Recombinant Cullin-1 is typically supplied as a solution in buffers containing HEPES, NaCl, DTT, and glycerol. It is shipped with dry ice and should be stored at -70°C to maintain stability. Repeated freeze-thaw cycles should be avoided to preserve protein integrity .