CTSL1 Antibody
Description
Product Specs
Product Science Overview
Cathepsin L is a member of the papain superfamily of cysteine proteases, which are enzymes that break down proteins by cleaving peptide bonds. It is synthesized as an inactive proenzyme and becomes active upon cleavage of its propeptide. Cathepsin L is found in lysosomes, where it plays a crucial role in the degradation of intracellular and extracellular proteins .
Cathepsin L is involved in various physiological processes, including:
- Protein Degradation: It hydrolyzes a number of proteins, including the proform of urokinase-type plasminogen activator, which is activated by Cathepsin L cleavage .
- Immune Response: It proteolytically inactivates alpha 1-antitrypsin and secretory leucoprotease inhibitor, two major protease inhibitors of the respiratory tract .
- Disease Mechanisms: Cathepsin L has been implicated in the pathology of several diseases, including cancer, osteoporosis, and viral infections such as SARS-CoV-2 .
The Mouse Anti-Human Cathepsin L antibody is a monoclonal antibody that specifically detects human Cathepsin L. It is produced by immunizing mice with human Cathepsin L and then harvesting the antibody-producing cells from the mice. These cells are fused with myeloma cells to create hybridomas, which can be cultured to produce large quantities of the antibody .
The Mouse Anti-Human Cathepsin L antibody is used in various research applications, including:
- Western Blotting: It detects both the pro and active forms of recombinant human and mouse Cathepsin L in Western blots .
- Immunohistochemistry: It is used to detect Cathepsin L in tissue sections, such as human kidney tissue .
- Immunoprecipitation: It is used to isolate Cathepsin L from cell lysates or conditioned media .
The Mouse Anti-Human Cathepsin L antibody is highly specific for human Cathepsin L and does not cross-react with other related lysosomal cysteine proteases, such as Cathepsin B, C, L2, O, S, or X/Z/P . This specificity makes it a valuable tool for studying Cathepsin L in various biological contexts.
