CTHRC1 Human, Sf9
Sf9, Baculovirus cells.
Collagen triple helix repeat-containing protein 1 isoform 1, CTHRC1, Protein NMTC1.
Description
CTHRC1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 222 amino acids (31-243a.a.) and having a molecular mass of 24.1kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). CTHRC1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Collagen triple helix repeat-containing protein 1 isoform 1, CTHRC1, Protein NMTC1.
Sf9, Baculovirus cells.
ADPSEIPKGK QKAQLRQREV VDLYNGMCLQ GPAGVPGRDG SPGANGIPGT PGIPGRDGFK GEKGECLRES FEESWTPNYK QCSWSSLNYG IDLGKIAECT FTKMRSNSAL RVLFSGSLRL KCRNACCQRW YFTFNGAECS GPLPIEAIIY LDQGSPEMNS TINIHRTSSV EGLCEGIGAG LVDVAIWVGT CSDYPKGDAS TGWNSVSRII IEELPKHHHH HH.
Product Science Overview
Collagen Triple Helix Repeat Containing 1 (CTHRC1) is a protein encoded by the CTHRC1 gene in humans. This protein is known for its role in various physiological and pathological processes, including vascular remodeling and cancer progression. The recombinant form of this protein, produced in Sf9 Baculovirus cells, is utilized in research to understand its functions and potential therapeutic applications.
CTHRC1 is a glycosylated, secreted protein with a molecular mass of approximately 28 kDa . It contains a short collagen motif with 12 Gly-X-Y repeats, which is responsible for the trimerization of the protein . This trimerization renders the molecule susceptible to cleavage by collagenase . The recombinant form of CTHRC1 produced in Sf9 cells is a single, glycosylated polypeptide chain containing 222 amino acids (31-243 a.a.) and has a molecular mass of 24.1 kDa .
CTHRC1 acts as a negative regulator of collagen matrix deposition . It is transiently expressed in the arterial wall in response to injury, where it contributes to vascular remodeling by inhibiting collagen expression and deposition and promoting cell migration . CTHRC1 is also highly expressed in multiple human cancers, including pancreatic cancer, where it plays a significant role in the progression and metastasis of the disease by regulating the migration and adhesion of tumor cells . Additionally, CTHRC1 overexpression can be used as an independent prognostic marker in gastric cancer .
Mutations in the CTHRC1 gene have been associated with Barrett esophagus and esophageal adenocarcinoma . The protein is found qualitatively in the plasma of healthy human subjects and its levels are significantly elevated during pregnancy, in diabetes, in inflammatory and infectious conditions, and in subjects with acute myeloid leukemia . However, it is not elevated in subjects with solid cancers .
The recombinant form of CTHRC1 produced in Sf9 cells is used in various research applications, including blocking assays and control experiments . It is supplied as a sterile filtered colorless solution and is highly purified, with a purity greater than 90% as determined by SDS-PAGE . The protein is stored in phosphate-buffered saline (PBS) with 10% glycerol and is stable at 4°C for short-term use and at -20°C for long-term storage .
