CSTF1 Human, sf9
Cleavage Stimulation Factor Subunit 1, Cleavage Stimulation Factor, 3 Pre-RNA, Subunit 1, 50kD, Cleavage Stimulation Factor 50 KDa Subunit, CF-1 50 KDa Subunit, CSTF 50 KDa Subunit, CstF-50, Cleavage Stimulation Factor, 3 Pre-RNA, Subunit 1, 50kDa, Cleavage Stimulation Factor, 3 Pre-RNA, Subunit 1, CstFp50.
Greater than 90.0% as determined by SDS-PAGE.
Description
CSTF1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 437 amino acids (1-431a.a.) and having a molecular mass of 49.1kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
CSTF1 is expressed with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Cleavage stimulation factor subunit 1 (CSTF1) plays a crucial role in the polyadenylation and cleavage of pre-mRNAs at their 3' ends. It is one of three subunits that combine to form cleavage stimulation factor (CSTF). CSTF1 is structurally similar to mammalian G protein beta subunits, containing transducin-like repeats. This protein is essential for the interaction of CSTF with other factors, forming a stable complex on the pre-mRNA that is required for polyadenylation and 3'-end cleavage.
Recombinant human CSTF1, expressed in Sf9 insect cells using a baculovirus system, is a single, glycosylated polypeptide chain. This protein consists of 437 amino acids (residues 1-431 with a 6-His tag) and has a molecular weight of 49.1 kDa. On SDS-PAGE, it appears as a band at approximately 40-57 kDa. The protein is purified using proprietary chromatographic methods and is tagged with a 6-amino acid His tag at the C-terminus.
The CSTF1 protein solution has a concentration of 0.5 mg/ml and is supplied in Phosphate Buffered Saline (pH 7.4) containing 1 mM DTT and 20% glycerol.
The purity of CSTF1 is determined to be greater than 90% by SDS-PAGE analysis.
Cleavage Stimulation Factor Subunit 1, Cleavage Stimulation Factor, 3 Pre-RNA, Subunit 1, 50kD, Cleavage Stimulation Factor 50 KDa Subunit, CF-1 50 KDa Subunit, CSTF 50 KDa Subunit, CstF-50, Cleavage Stimulation Factor, 3 Pre-RNA, Subunit 1, 50kDa, Cleavage Stimulation Factor, 3 Pre-RNA, Subunit 1, CstFp50.
MYRTKVGLKD RQQLYKLIIS QLLYDGYISI ANGLINEIKP QSVCAPSEQL LHLIKLGMEN DDTAVQYAIG RSDTVAPGTG IDLEFDADVQ TMSPEASEYE TCYVTSHKGP CRVATYSRDG QLIATGSADA SIKILDTERM LAKSAMPIEV MMNETAQQNM ENHPVIRTLY DHVDEVTCLA FHPTEQILAS GSRDYTLKLF DYSKPSAKRA FKYIQEAEML RSISFHPSGD FILVGTQHPT LRLYDINTFQ CFVSCNPQDQ HTDAICSVNY NSSANMYVTG SKDGCIKLWD GVSNRCITTF EKAHDGAEVC SAIFSKNSKY ILSSGKDSVA KLWEISTGRT LVRYTGAGLS GRQVHRTQAV FNHTEDYVLL PDERTISLCC WDSRTAERRN LLSLGHNNIV RCIVHSPTNP GFMTCSDDFR ARFWYRRSTT DHHHHHH.
Product Science Overview
CSTF1 contains transducin-like repeats, similar to the mammalian G protein beta subunits . These repeats are essential for the protein’s function in the CSTF complex. The CSTF complex is necessary for the proper processing of pre-mRNA molecules, which involves the addition of a poly(A) tail at the 3’ end of the mRNA precursor. This polyadenylation process is critical for the stability, export, and translation of mRNA molecules.
The recombinant form of CSTF1, produced in sf9 cells, is used for various research and experimental purposes. Sf9 cells are derived from the fall armyworm (Spodoptera frugiperda) and are commonly used in the baculovirus expression system for producing recombinant proteins. This system is advantageous because it allows for high-level expression of proteins with proper post-translational modifications.
Recombinant CSTF1 is used in studies related to mRNA processing, gene expression regulation, and the mechanisms of polyadenylation. It is also utilized in biochemical assays to understand the interactions and functions of the CSTF complex and its role in mRNA maturation.
Understanding the function and regulation of CSTF1 is vital for comprehending the broader mechanisms of gene expression and mRNA processing. Research on CSTF1 and its recombinant forms can provide insights into various cellular processes and contribute to the development of therapeutic strategies for diseases related to mRNA processing defects.
For more detailed information, you can refer to the ProSpec and Biocompare websites.
