Cystatin 7, also known as Cystatin F, is a member of the cystatin superfamily, which consists of natural cysteine protease inhibitors. These inhibitors are present in a wide variety of organisms and play crucial roles in regulating protease activity. Cystatin 7 is particularly notable for its involvement in immune regulation and its potential implications in cancer research.
Cystatin 7 is a type 2 cystatin, which means it belongs to a class of cysteine proteinase inhibitors found in various human fluids and secretions. The protein is glycosylated and has a molecular weight of approximately 15.2 kDa, although it is observed to be around 25 kDa due to glycosylation . The recombinant form of Cystatin 7 is typically expressed in HEK293 cells, a human embryonic kidney cell line commonly used for protein production .
Cystatin 7 is primarily expressed in hematopoietic cells, with the highest levels observed in monocytes, dendritic cells, and certain types of T-cells . Its expression is up-regulated during the maturation of dendritic cells, suggesting a specific role in immune regulation . Cystatin 7 functions as an inhibitor of cysteine proteases, enzymes that break down proteins by cleaving peptide bonds. By inhibiting these proteases, Cystatin 7 helps regulate various physiological processes, including immune responses and inflammation.
Cystatin 7 has been observed in various human cancer cell lines, indicating its potential role in cancer progression and metastasis . The protein is produced as a dimer, an inactive form that becomes an active cathepsin inhibitor upon chemical reduction . This activation mechanism suggests that Cystatin 7 could be involved in controlling protease activity in the tumor microenvironment, thereby influencing cancer cell behavior.
Recombinant Cystatin 7 is widely used in research to study its role in immune regulation and cancer. The protein is typically provided as a lyophilized powder, which can be reconstituted for experimental use . It is essential to handle and store the protein properly to maintain its stability and activity. Researchers often use Cystatin 7 to investigate its interactions with cysteine proteases and to explore its potential as a therapeutic target for immune-related disorders and cancer.