CST6 Human, Active

Cystatin E/M, BioActive Human Recombinant
Cat. No.
BT25211
Source
E.coli.
Synonyms

Cystatin E/M, cystatin 6, Cystatin M, Cystatin-E, Cysteine proteinase inhibitor.

Appearance
Sterile Filtered colorless solution.
Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CST6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 142 amino acids (29-149 a.a.) and having a molecular mass of 15.9kDa.
CST6 is fused to a 21 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Cystatin E/M, also known as CST6, belongs to the cystatin type 2 family. While some members of this family act as cysteine protease inhibitors, CST6 specifically regulates cathepsin B inhibitors and not cathepsin C. It plays a role in various biological processes including osteogenesis, bone resorption, insulin regulation, systemic inflammation response, and hepatocyte growth factor receptor activity.
Description
Recombinant Human CST6, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 142 amino acids (residues 29-149). With a molecular weight of 15.9 kDa, it includes a 21 amino acid His tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The CST6 solution is provided at a concentration of 0.5 mg/mL in a buffer consisting of 10% glycerol, 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein such as HSA or BSA (0.1%) is advised for long-term storage. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of CST6 is determined to be greater than 90.0% based on SDS-PAGE analysis.
Biological Activity
CST6 exhibits an IC50 value of less than 10 nM. Its inhibitory effect on the protease activity of papain was assessed using a fluorometric assay with Z-FR-AMC as the substrate. The assay was conducted at pH 7.5 and a temperature of 25°C.
Synonyms

Cystatin E/M, cystatin 6, Cystatin M, Cystatin-E, Cysteine proteinase inhibitor.

Source
E.coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MRPQERMVGE LRDLSPDDPQ VQKAAQAAVA SYNMGSNSIY YFRDTHIIKA QSQLVAGIKY FLTMEMGSTD CRKTRVTGDH VDLTTCPLAA GAQQEKLRCD FEVLVVPWQN SSQLLKHNCV QM

Product Science Overview

Introduction

Cystatin E/M, also known as cystatin 6, is a member of the cystatin superfamily, specifically family 2. This family of proteins is known for their role as cysteine protease inhibitors. Cystatin E/M is encoded by the CST6 gene and has been studied for its inhibitory effects on enzymes such as papain and cathepsin B .

Structure and Properties

Recombinant human Cystatin E/M is typically produced in a mouse myeloma cell line (NS0) and is characterized by a molecular mass of approximately 15 kDa. The protein is often tagged with a C-terminal 10-His tag to facilitate purification and detection . The recombinant form is highly purified, with a purity greater than 95%, as determined by SDS-PAGE and visualized with silver staining .

Biological Activity

The primary biological function of Cystatin E/M is to inhibit cysteine proteases. It has been shown to effectively inhibit the cleavage of a fluorogenic peptide substrate by papain, with an IC50 value of approximately 7.0 nM . This inhibition is crucial for regulating protease activity in various physiological processes.

Expression and Tissue Distribution

Cystatin E/M mRNA has been detected in a wide range of tissues, indicating its broad physiological relevance . The expression patterns suggest that it plays a significant role in maintaining protease balance in different cellular environments.

Applications

Recombinant human Cystatin E/M is used in various research applications, including:

  • Protease inhibition studies: To understand the regulatory mechanisms of cysteine proteases.
  • Cell culture: As a supplement to study the effects of protease inhibition in different cell types.
  • Enzyme assays: To quantify the inhibitory activity of Cystatin E/M against specific proteases .
Storage and Stability

The recombinant protein is typically lyophilized and should be reconstituted in a suitable buffer before use. It is recommended to store the protein at -20 to -70°C to maintain its stability and avoid repeated freeze-thaw cycles .

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CST6 Human
Cystatin E/M Human Recombinant
Cat. No.
BT25138
Source
E.coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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