CSRP2 Human

Cysteine and Glycine-rich Protein 2 Human Recombinant
Cat. No.
BT3012
Source
E.coli.
Synonyms
Cysteine and glycine-rich protein 2, Smooth muscle cell LIM protein, LIM domain only 5 smooth muscle, SmLIM, LMO5, CRP2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CSRP2 Human Recombinant produced in E. coli is a single polypeptide chain containing 217 amino acids (1-193) and having a molecular mass of 23.5kDa.
CSRP2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
CSRP2, a member of the CSRP family, plays a crucial role in development and cellular differentiation due to its two LIM zinc-binding domains. This protein is also involved in the formation of the embryonic vascular system.
Description
Recombinant human CSRP2, produced in E. coli, is a single polypeptide chain of 217 amino acids (residues 1-193) with a molecular weight of 23.5 kDa. It includes a 24 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The CSRP2 solution is provided at a concentration of 0.5 mg/mL in a buffer containing 20 mM Tris-HCl (pH 8.0), 200 mM NaCl, 2 mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended periods, freeze at -20°C. Adding a carrier protein like 0.1% HSA or BSA is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity exceeds 90% as determined by SDS-PAGE analysis.
Synonyms
Cysteine and glycine-rich protein 2, Smooth muscle cell LIM protein, LIM domain only 5 smooth muscle, SmLIM, LMO5, CRP2.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMPVWGG GNKCGACGRT VYHAEEVQCD GRSFHRCCFL CMVCRKNLDS TTVAIHDEEI YCKSCYGKKY GPKGYGYGQG AGTLNMDRGE RLGIKPESVQ PHRPTTNPNT SKFAQKYGGA EKCSRCGDSV YAAEKIIGAG KPWHKNCFRC AKCGKSLEST TLTEKEGEIY CKGCYAKNFG PKGFGYGQGA GALVHAQ

Product Science Overview

Gene and Protein Structure

The CSRP2 gene is located on chromosome 12 in humans . It encodes a protein that contains two copies of the cysteine-rich amino acid sequence motif (LIM) with putative zinc-binding activity . This structure is essential for the protein’s function in regulating ordered cell growth and differentiation .

Function and Biological Role

CSRP2 is involved in several critical biological processes. It plays a role in the development of the embryonic vascular system and is drastically down-regulated in response to platelet-derived growth factor (PDGF-BB) or cell injury, which promotes smooth muscle cell proliferation and dedifferentiation . This suggests that CSRP2 may act as a regulatory protein in smooth muscle cells, contributing to the maintenance of their differentiated state .

Clinical Significance

Mutations or alterations in the CSRP2 gene have been associated with various diseases. For example, CSRP2 is linked to Chromosome 6Q24-Q25 Deletion Syndrome, a genetic disorder characterized by developmental delays and other abnormalities . Additionally, CSRP2 has been identified as a novel marker of hepatic stellate cells and a binding partner of the protein inhibitor of activated STAT1, indicating its potential role in liver function and disease .

Research and Applications

The recombinant form of CSRP2 is used in various research applications to study its function and role in different biological processes. Understanding the mechanisms by which CSRP2 regulates cell growth and differentiation can provide insights into the development of therapeutic strategies for diseases associated with its dysfunction .

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